Human TCN2 ELISA Kit | TCN2 elisa kit

Human TCN2 PicoKine ELISA Kit

Cat. Num. AAA1752643

• Gene Names: TCN2; II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
• Reactivity: Human
• Synonyms: TCN2; Human TCN2 PicoKine ELISA Kit; Human TCN2; transcobalamin 2; Transcobalamin-2; TC-2; Transcobalamin II; TC II; TCII; TC2; TCN2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human TCN2
• Sequence Length: 400

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and urine
• Detection Range: 0.78ng/ml-50ng/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TCN2 elisa kit

Principle of the Assay: The Picokine™ Human TCN2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human TCN2 with a 96-well strip plate that is pre-coated with antibody specific for TCN2. The detection antibody is a biotinylated antibody specific for TCN2. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human TCN2 with immunogen: Expression system for standard: NSO, Immunogen sequence: E19-W427. The kit is analytically validated with ready to use reagents. To measure Human TCN2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human TCN2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TCN2 in the sample.!!Background/Introduction: TCN2 (Transcobalamin II; also TC-2 or TC) is a 42-44 kDa, monomeric, secreted member of the eukaryotic cobalamin transport family of molecules. This gene is mapped to 22q12.2. This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 296080704
• NCBI GeneID: 6948
• NCBI Accession #: NP_001171655.1
• NCBI GenBank Nucleotide #: NP_001171655.1
• UniProt Accession #: P20062
• NCBI Official Full Name: transcobalamin-2 isoform 2
• NCBI Official Synonym Full Names: transcobalamin 2
• NCBI Official Symbol: TCN2
• NCBI Official Synonym Symbols: II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
• NCBI Protein Information: transcobalamin-2
• UniProt Protein Name: Transcobalamin-2
• Protein Family: Transcobalamin
• UniProt Gene Name: TCN2
• UniProt Synonym Gene Names: TC2; TC-2; TC II; TCII
• UniProt Entry Name: TCO2_HUMAN

NCBI Description

This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

TCN2: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells. Defects in TCN2 are the cause of transcobalamin II deficiency (TCN2 deficiency). This results in various forms of anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: extracellular space; lysosomal lumen; extracellular region; endosome

Molecular Function: metal ion binding; cobalamin binding

Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process

Disease: Transcobalamin Ii Deficiency