IL-2RB/CD122 recombinant protein

IL-2RB/CD122 Recombinant Protein

Cat. Num. AAA3003758

• Gene Names: IL2RB; CD122; IL15RB; P70-75
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Synonyms: IL-2RB/CD122; IL-2RB/CD122 Recombinant Protein; IL-2RB/CD122 recombinant protein

• Host: E Coli
• Purity/Purification: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Form/Format: PBS, 4M Urea, PH7.4
• Concentration: >=0.5mg/ml (varies by lot)
• Sequence: AVNGTSQFTCFYNSRANISCVWSQDGALQDTSCQVHAWPDRRRWNQTCELLPVSQASWACNLILGAPDSQKLTTVDIVTLRVLCREGVRWRVMAIQDFKPFENLRLMAPISLQVVHVETHRCNISWEISQASHYFERHLEFEARTLSPGHTWEEAPLLTLKQKQEWICLETLTPDTQYEFQVRVKPLQGEFTTWSPWSQPLAFRTKPAALGKDT

• Tag: His-tag in C-terminal
• Expression Vector: pet-22b(+)
• BP: 654
• Restriction Site: NdeI-XhoI
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Testing Data

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Related Product Information for IL-2RB/CD122 recombinant protein

Background: Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation. IL-2 also promotes peripheral development of regulatory T cells (Tregs). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells. The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Ralpha, IL-2Rbeta, and the common gamma chain, gammac. IL-2Ralpha binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rbeta and gammac subunits. IL-15 also binds to IL-2Rbeta. gammac is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21. Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways. IL-2Rbeta, also known as CD122, is a type I transmembrane protein that is expressed by natural killer cells and certain subsets of T cells in the periphery. IL-2Rbeta surface expression is upregulated following antigen-induced activation. A heteromeric complex composed of IL-2Rbeta and gammac forms the intermediate affinity IL-2 receptor whereas a complex comprised of all three chains forms the high affinity receptor. Signaling through IL-2Rbeta is mediated by Jak1, which phosphorylates numerous tyrosine residues in the cytoplasmic tail to promote the recruitment of the adaptor Shc to IL-2Rbeta and enhance IL-2 receptor signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 4504665
• NCBI GeneID: 3560
• NCBI Accession #: NP_000869.1
• NCBI GenBank Nucleotide #: NM_000878.3
• UniProt Accession #: P14784; B2R765
• Molecular Weight: 61,117 Da
• NCBI Official Full Name: interleukin-2 receptor subunit beta
• NCBI Official Synonym Full Names: interleukin 2 receptor, beta
• NCBI Official Symbol: IL2RB
• NCBI Official Synonym Symbols: CD122; IL15RB; P70-75
• NCBI Protein Information: interleukin-2 receptor subunit beta; p75; IL-2RB; CD122 antigen; IL-2R subunit beta; IL-2 receptor subunit beta; interleukin 15 receptor, beta; high affinity IL-2 receptor beta subunit; high affinity IL-2 receptor subunit beta
• UniProt Protein Name: Interleukin-2 receptor subunit beta
• UniProt Gene Name: IL2RB
• UniProt Synonym Gene Names: IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; p75
• UniProt Entry Name: IL2RB_HUMAN

NCBI Description

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. [provided by RefSeq, Jul 2008]

Uniprot Description

IL2RB: Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Interacts with SHB upon interleukin stimulation. Interacts with HTLV-1 accessory protein p12I. Belongs to the type I cytokine receptor family. Type 4 subfamily.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: interleukin-2 receptor activity; interleukin-2 binding

Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; protein complex assembly; signal transduction; negative regulation of apoptosis