TCN2 active protein

TCN2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120359

• Gene Names: TCN2; II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TCN2; TCN2 Protein; Human; Recombinant (His Tag); Human TCN2 Protein (His Tag); D22S676 Protein; D22S750 Protein; II Protein; TC Protein; TC-2 Protein; TC2 Protein; TCII Protein; transcobalamin II; TCN2 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Trp427

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human TCN2 (NP_000346.2)(Met 1-Trp 427) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its binding ability in a functional ELISA.; 2. Immobilized human TCN2-His at 10ug/mL (100uL/well) can bind biotinylated mouse CD320-His. The EC50 of biotinylated mouse CD320-His is 18-42ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TCN2 active protein

Background: Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 2 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.

References

Rothenberg, S. P. et al., 1995, Baillieres Clin Haematol. 8 (3):499-514. Bibi, H. et al., 1999, J Inherit Metab Dis. 22 (7):765-772. Seetharam,B. et al.,2000, Vitam Horm. 59 :337-366.

NCBI and Uniprot Product Information

• NCBI GI #: 21071010
• NCBI GeneID: 6948
• NCBI Accession #: NP_000346.2
• NCBI GenBank Nucleotide #: NM_000355.3
• UniProt Accession #: P20062; Q96FD4; Q9BVI8; Q9UCI5; Q9UCI6; Q9UDM0; AAB25526
• Molecular Weight: 44,421 Da
• NCBI Official Full Name: transcobalamin-2 isoform 1
• NCBI Official Synonym Full Names: transcobalamin 2
• NCBI Official Symbol: TCN2
• NCBI Official Synonym Symbols: II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
• NCBI Protein Information: transcobalamin-2
• UniProt Protein Name: Transcobalamin-2
• Protein Family: Transcobalamin
• UniProt Gene Name: TCN2
• UniProt Synonym Gene Names: TC2; TC-2; TC II; TCII
• UniProt Entry Name: TCO2_HUMAN

NCBI Description

This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

TCN2: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells. Defects in TCN2 are the cause of transcobalamin II deficiency (TCN2 deficiency). This results in various forms of anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: extracellular space; lysosomal lumen; extracellular region; endosome

Molecular Function: metal ion binding; cobalamin binding

Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process

Disease: Transcobalamin Ii Deficiency