Western Blot (WB)
(Western blot analysis of JHDM1D Antibody (Center) in 293 cell line lysates (35ug/lane).JHDM1D (arrow) was detected using the purified Pab.)
Rabbit anti-Human JHDM1D Polyclonal Antibody | anti-JHDM1D antibody
JHDM1D, ID (JHDM1D, KDM7, KIAA1718, Lysine-specific demethylase 7, JmjC domain-containing histone demethylation protein 1D) (APC)
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of JHDM1D Antibody (Center) in 293 cell line lysates (35ug/lane).JHDM1D (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of JHDM1D Antibody (Center) in 293 cell line lysates (35ug/lane).JHDM1D (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(JHDM1D Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the JHDM1D Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(JHDM1D Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the JHDM1D Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(JHDM1D Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(JHDM1D Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
JHDM1D: Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate. Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.-
Chromosomal Location of Human Ortholog: 7q34
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: zinc ion binding; histone demethylase activity (H3-K36 specific); iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; histone demethylase activity (H3-K9 specific); methylated histone residue binding
Biological Process: establishment and/or maintenance of chromatin architecture; histone H3-K9 demethylation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; midbrain development
Research Articles on JHDM1D
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Product Notes
The JHDM1D jhdm1d (Catalog #AAA6311588) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JHDM1D, ID (JHDM1D, KDM7, KIAA1718, Lysine-specific demethylase 7, JmjC domain-containing histone demethylation protein 1D) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JHDM1D can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the JHDM1D jhdm1d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JHDM1D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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