Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Synaptotagmin-4 (SYT4) Standard Curve.)
Human Synaptotagmin-4 (SYT4) ELISA Kit | SYT4 elisa kit
Human Synaptotagmin-4 (SYT4) ELISA Kit
Gene Names
SYT4; HsT1192
Reactivity
Human
Synonyms
Synaptotagmin-4 (SYT4); Human Synaptotagmin-4 (SYT4) ELISA Kit; SYT4; HsT1192; KIAA1342; synaptotagmin 4; SYT4 elisa kit
Reactivity
Human
Specificity
Human Synaptotagmin-4 (SYT4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SYT4. No significant cross-reactivity or interference between Human SYT4 and analogues was observed.
Sequence Length
425
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Synaptotagmin-4 (SYT4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Synaptotagmin-4 (SYT4) Standard Curve.)
Related Product Information for SYT4 elisa kit
Description: This Human Synaptotagmin-4 (SYT4) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT4 in samples. An antibody specific for SYT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SYT4 (Synaptotagmin 4) is a Protein Coding gene. GO annotations related to this gene include calcium ion binding and syntaxin binding. An important paralog of this gene is SYT11. Synaptotagmin 4 may be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. It plays a role in dendrite formation by melanocytes
Background: SYT4 (Synaptotagmin 4) is a Protein Coding gene. GO annotations related to this gene include calcium ion binding and syntaxin binding. An important paralog of this gene is SYT11. Synaptotagmin 4 may be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. It plays a role in dendrite formation by melanocytes
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
synaptotagmin-4
NCBI Official Synonym Full Names
synaptotagmin 4
NCBI Official Symbol
SYT4
NCBI Official Synonym Symbols
HsT1192
NCBI Protein Information
synaptotagmin-4
UniProt Protein Name
Synaptotagmin-4
Protein Family
UniProt Gene Name
SYT4
UniProt Synonym Gene Names
KIAA1342; SytIV
UniProt Entry Name
SYT4_HUMAN
Uniprot Description
SYT4: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Belongs to the synaptotagmin family.
Protein type: Vesicle; Calcium-binding; Membrane protein, integral
Chromosomal Location of Human Ortholog: 18q12.3
Cellular Component: neuron projection; synaptic vesicle membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; integral to membrane; plasma membrane; cell junction
Molecular Function: clathrin binding; SNARE binding; phosphatidylserine binding; transporter activity; calcium ion binding
Biological Process: negative regulation of vesicle fusion; exocytosis; negative regulation of calcium ion-dependent exocytosis; neurotransmitter secretion
Research Articles on SYT4
Similar Products
Product Notes
The Human SYT4 syt4 (Catalog #AAA9714101) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714101 ELISA Kit recognizes Human SYT4. It is sometimes possible for the material contained within the vial of "Synaptotagmin-4 (SYT4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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