Rabbit anti-Human SYT4 Polyclonal Antibody | anti-SYT4 antibody

SYT4, NT (SYT4, KIAA1342, Synaptotagmin-4, Synaptotagmin IV) (APC)

Cat. Num. AAA6353018

• Gene Names: SYT4; HsT1192
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SYT4; Polyclonal Antibody; NT (SYT4; KIAA1342; Synaptotagmin-4; Synaptotagmin IV) (APC); Synaptotagmin IV; anti-SYT4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SYT4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SYT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-85 amino acids from the N-terminal region of human SYT4.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SYT4 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the SYT4 antibody detected the SYT4 protein (arrow).)

Related Product Information for anti-SYT4 antibody

SYT4 may be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.

Product Categories/Family for anti-SYT4 antibody

Antibodies; Syntaxin; Synaptotagmin; Synaptophysin

References

Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009). Sonuga-Barke, E.J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1359-1368 (2008): Ferguson, G.D., et al. J. Biol. Chem. 275(47):36920-36926(2000). Mizutani, A., et al. J. Biol. Chem. 275(13):9823-9831(2000). Thomas, D.M., et al. Mol. Biol. Cell 10(7):2285-2295(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 24308219
• NCBI GeneID: 6860
• NCBI Accession #: NP_065834.1
• NCBI GenBank Nucleotide #: NM_020783.3
• UniProt Accession #: Q9H2B2; Q9P2K4; B4DEU3
• Molecular Weight: 46,064 Da
• NCBI Official Full Name: synaptotagmin-4
• NCBI Official Synonym Full Names: synaptotagmin IV
• NCBI Official Symbol: SYT4
• NCBI Official Synonym Symbols: HsT1192
• NCBI Protein Information: synaptotagmin-4; synaptotagmin 4; sytIV
• UniProt Protein Name: Synaptotagmin-4
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT4
• UniProt Synonym Gene Names: KIAA1342; SytIV
• UniProt Entry Name: SYT4_HUMAN

Uniprot Description

SYT4: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Belongs to the synaptotagmin family.

Protein type: Vesicle; Calcium-binding; Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q12.3

Cellular Component: neuron projection; synaptic vesicle membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; integral to membrane; plasma membrane; cell junction

Molecular Function: clathrin binding; SNARE binding; phosphatidylserine binding; transporter activity; calcium ion binding

Biological Process: negative regulation of vesicle fusion; exocytosis; negative regulation of calcium ion-dependent exocytosis; neurotransmitter secretion