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Western Blot (WB) (Western Blot of Visfatin Antibody (MBS540842) with Visfatin positive Control (MBS543377). Apparent Molecular weight is 59 kDa. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)

Visfatin Blocking Peptide | NAMPT blocking peptide

Visfatin Peptide

Gene Names
NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
Applications
Immunocompetition, Immunodepletion
Synonyms
Visfatin; Visfatin Peptide; 1110035O14Rik; AI314458; AI480535; DKFZP666B131; EC 2.4.2.12; MGC117256; NAmPRTase; Nampt; Nicotinamide phosphoribosyltransferase; PBEF; PBEF1; Pre B cell colony enhancing factor; Pre B cell enhancing factor; VF; Visfatin antibody; NAMPT blocking peptide
Ordering
For Research Use Only!
Form/Format
Antigenic Blocking Peptide
Concentration
Quantity: 250 ug; Volume: 100 ul (varies by lot)
Sequence Length
491
Applicable Applications for NAMPT blocking peptide
Immunocompetition, Immunodepletion
Application Notes
Western Blot: 1:500
Immunogen
C-epitope synthetic peptide taken within amino acid region 400-490 on human Visfatin protein.
Expression
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
Determinant
C-epitope
Preparation and Storage
-20 degree C for long term storage

Western Blot (WB)

(Western Blot of Visfatin Antibody (MBS540842) with Visfatin positive Control (MBS543377). Apparent Molecular weight is 59 kDa. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)

Western Blot (WB) (Western Blot of Visfatin Antibody (MBS540842) with Visfatin positive Control (MBS543377). Apparent Molecular weight is 59 kDa. Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
Related Product Information for NAMPT blocking peptide
Visfatin (also known as Nicotinamide Phosphoribosyltransferase / NAMPT / Pre-B cell colony-enhancing factor / PBEF) is a ubiquitous 52 kDa adipokine secreted by adipose tissue. Visfatin is the rate-limiting component and intracellularly it acts as a cytosolic enzyme involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+, 1 ). Although it lacks a signal sequence, Visfatin is secreted by visceral adipose and functions as noncompetitive insulin mimetic and functions in the cytoplasm to regulate energy metabolism during stress responses, lowers blood glucose, improve insulin sensitivity and immune activation. Visfatin was first identified as a factor that enhanced the differentiation of B-cells. It is a secreted factor that is highly expressed in adipocytes. Visfatin is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity and type 2 diabetes mellitus (2). Visfatin is expressed in large amounts in bone marrow, liver tissue, and muscle. It is also present in heart, placenta, lung, and kidney tissues. This gene has a pseudo gene on chromosome 10. Visfatin-selective antibodies were generated against ulJique N-terminal and C-terminal peptides characteristics of the human VISFATIN protein. FabGennix has generated highly specific rabbit VISFATIN polyclonal antibodies utilizing mostly N-terminal and C-terminal sequences. These antibodies have been fully characterized for cross reactivity with other cellular proteins. FabGennix has produced antibodies to multiple epitopes on the same protein that will facilitate studies utilizing interspecies cross reactivity. Western blot positive control samples in "ready-to-use" SDS-PAGE sample buffer and antigenic blocking peptide for Visfatin are available. The Visfatin positive control appears as a diffuse band around 60 kDa and co-migrates with rat adipocyte membrane Visfatin protein on a 10% SDS Page. All antibodies can be conjugated to fluorophores and other secondary enzymes as an additional service.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nicotinamide phosphoribosyltransferase
NCBI Official Synonym Full Names
nicotinamide phosphoribosyltransferase
NCBI Official Symbol
NAMPT
NCBI Official Synonym Symbols
VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
NCBI Protein Information
nicotinamide phosphoribosyltransferase
UniProt Protein Name
Nicotinamide phosphoribosyltransferase
UniProt Gene Name
NAMPT
UniProt Synonym Gene Names
PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
UniProt Entry Name
NAMPT_HUMAN

NCBI Description

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

Uniprot Description

PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis.

Protein type: Transferase; Cytokine; EC 2.4.2.12; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: cell junction; cytosol; extracellular space; nucleoplasm

Molecular Function: cytokine activity; drug binding; nicotinamide phosphoribosyltransferase activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; protein binding; protein homodimerization activity

Biological Process: cell-cell signaling; circadian regulation of gene expression; circadian rhythm; female pregnancy; insulin receptor signaling pathway; NAD biosynthetic process; nicotinamide metabolic process; positive regulation of cell proliferation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; response to organic cyclic substance; signal transduction

Research Articles on NAMPT

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Product Notes

The NAMPT nampt (Catalog #AAA544653) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Visfatin can be used in a range of immunoassay formats including, but not limited to, Immunocompetition, Immunodepletion. Western Blot: 1:500. Researchers should empirically determine the suitability of the NAMPT nampt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Visfatin, Blocking Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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