Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Surfactant-associated protein 2 (SFTA2) Standard Curve.)
Human Surfactant-Associated Protein 2 (SFTA2) ELISA Kit | SFTA2 elisa kit
Human Surfactant-Associated Protein 2 (SFTA2) ELISA Kit
Gene Names
SFTA2; SP-G; SFTPG; UNQ541; GSGL541
Reactivity
Human
Synonyms
Surfactant-Associated Protein 2 (SFTA2); Human Surfactant-Associated Protein 2 (SFTA2) ELISA Kit; SFTA2; DADB-160J14.6; GSGL541; SFTPG; UNQ541; surfactant associated protein G; SFTA2 elisa kit
Reactivity
Human
Specificity
Human Surfactant-associated protein 2 (SFTA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SFTA2. No significant cross-reactivity or interference between Human SFTA2 and analogues was observed.
Sequence Length
78
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Surfactant-associated protein 2 (SFTA2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Surfactant-associated protein 2 (SFTA2) Standard Curve.)
Related Product Information for SFTA2 elisa kit
Description: This Human Surfactant-associated protein 2 (SFTA2) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTA2 in samples. An antibody specific for SFTA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFTA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SFTPGis an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period.
Background: SFTPGis an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
surfactant associated protein G
NCBI Official Synonym Full Names
surfactant associated 2
NCBI Official Symbol
SFTA2
NCBI Official Synonym Symbols
SP-G; SFTPG; UNQ541; GSGL541
NCBI Protein Information
surfactant-associated protein 2
UniProt Protein Name
Surfactant-associated protein 2
Protein Family
UniProt Gene Name
SFTA2
UniProt Synonym Gene Names
SFTPG; SP-G
UniProt Entry Name
SFTA2_HUMAN
Uniprot Description
SFTA2:
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 6p21.33
Cellular Component: extracellular region
Research Articles on SFTA2
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Product Notes
The Human SFTA2 sfta2 (Catalog #AAA9714342) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714342 ELISA Kit recognizes Human SFTA2. It is sometimes possible for the material contained within the vial of "Surfactant-Associated Protein 2 (SFTA2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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