Human Spondin 1 (SPON1) ELISA Kit | SPON1 elisa kit

Human Spondin 1 (SPON1) ELISA Kit

Cat. Num. AAA450980

• Gene Names: SPON1; f-spondin; VSGP/F-spondin
• Reactivity: Human
• Synonyms: Spondin 1 (SPON1); Human Spondin 1 (SPON1) ELISA Kit; F-spondin; Vascular smooth muscle cell growth-promoting factor; VSGP; SPON1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SPON1. No significant cross-reactivity or interference between SPON1 and analogues was observed.
• Sequence Length: 807

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.212ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SPON1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SPON1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPON1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SPON1 in human serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPON1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SPON1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPON1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SPON1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 110347423
• NCBI GeneID: 10418
• NCBI Accession #: NP_006099.2
• NCBI GenBank Nucleotide #: NM_006108.3
• UniProt Accession #: Q9HCB6; O94862; Q8NCD7; Q8WUR5; A8K6W5
• Molecular Weight: 90,973 Da
• NCBI Official Full Name: spondin-1
• NCBI Official Synonym Full Names: spondin 1
• NCBI Official Symbol: SPON1
• NCBI Official Synonym Symbols: f-spondin; VSGP/F-spondin
• NCBI Protein Information: spondin-1
• UniProt Protein Name: Spondin-1
• Protein Family: Spondin
• UniProt Gene Name: SPON1
• UniProt Synonym Gene Names: KIAA0762; VSGP

Uniprot Description

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (). Major factor for vascular smooth muscle cell.