Human Integrin Alpha 10 (ITGa10) ELISA Kit | ITGa10 elisa kit

Human Integrin Alpha 10 (ITGa10) High Sensitive ELISA Kit

Cat. Num. AAA2708096

• Gene Names: ITGA10; PRO827
• Reactivity: Human
• Applications: ELISA
• Synonyms: Integrin Alpha 10 (ITGa10); Human Integrin Alpha 10 (ITGa10) High Sensitive ELISA Kit; Integrin Alpha 10; ITG-A10; ITGa10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Integrin Alpha 10 (ITGa10).; No significant cross-reactivity or interference between High Sensitive Integrin Alpha 10 (ITGa10) and analogues was observed.
• Sequence Length: 1167

• Applicable Applications for ITGa10 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 31.2-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 12.2pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Integrin Alpha 10 (ITGa10) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Integrin Alpha 10 (ITGa10) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Integrin Alpha 10 (ITGa10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Integrin Alpha 10 (ITGa10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Integrin Alpha 10 (ITGa10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Integrin Alpha 10 (ITGa10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 38569398
• NCBI GeneID: 8515
• NCBI Accession #: NP_003628.2
• NCBI GenBank Nucleotide #: NP_003628.2
• UniProt Accession #: O75578
• NCBI Official Full Name: integrin alpha-10 isoform 1
• NCBI Official Synonym Full Names: integrin subunit alpha 10
• NCBI Official Symbol: ITGA10
• NCBI Official Synonym Symbols: PRO827
• NCBI Protein Information: integrin alpha-10
• UniProt Protein Name: Integrin alpha-10
• Protein Family: Integrin
• UniProt Gene Name: ITGA10
• UniProt Entry Name: ITA10_HUMAN

NCBI Description

Integrins are integral transmembrane glycoproteins composed of noncovalently linked alpha and beta chains. They participate in cell adhesion as well as cell-surface mediated signalling. This gene encodes an integrin alpha chain and is expressed at high levels in chondrocytes, where it is transcriptionally regulated by AP-2epsilon and Ets-1. The protein encoded by this gene binds to collagen. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]