Human SPINK5 ELISA Kit | SPINK5 elisa kit

Human SPINK5 PicoKine ELISA Kit

Cat. Num. AAA1752648

• Gene Names: SPINK5; NS; NETS; LEKTI; LETKI; VAKTI
• Reactivity: Human
• Synonyms: SPINK5; Human SPINK5 PicoKine ELISA Kit; Human SPINK5; serine peptidase inhibitor; Kazal type 5; Serine protease inhibitor Kazal-type 5; Lympho-epithelial Kazal-type-related inhibitor; LEKTI; SPINK5 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human SPINK5
• Sequence Length: 1094

• Samples: Cell culture supernatants, serum and plasma (heparin, edta, citrate)
• Detection Range: 312 pg/ml - 20,000 pg/ml
• Sensitivity: <50 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPINK5 elisa kit

Principle of the Assay: The Picokine™ Human SPINK5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human SPINK5 with a 96-well strip plate that is pre-coated with antibody specific for SPINK5. The detection antibody is a biotinylated antibody specific for SPINK5. The capture antibody is a monoclonal antibody from mouse and the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human SPINK5 with immunogen: Expression system for standard: NS0; Immunogen sequence: K23-E1064. The kit is analytically validated with ready-to-use reagents. To measure Human SPINK5, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human SPINK5 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human SPINK5 in the sample.

Background/Introduction: Lympho-epithelial Kazal-type-related inhibitor (LEKTI) also known as serine protease inhibitor Kazal-type 5 (SPINK5) is a protein that in humans is encoded by the SPINK5 gene. This gene is mapped to 5q32. This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 189163501
• NCBI GeneID: 11005
• NCBI Accession #: NP_001121170.1
• NCBI GenBank Nucleotide #: NP_001121170.1
• UniProt Accession #: Q9NQ38
• NCBI Official Full Name: serine protease inhibitor Kazal-type 5 isoform a preproprotein
• NCBI Official Synonym Full Names: serine peptidase inhibitor, Kazal type 5
• NCBI Official Symbol: SPINK5
• NCBI Official Synonym Symbols: NS; NETS; LEKTI; LETKI; VAKTI
• NCBI Protein Information: serine protease inhibitor Kazal-type 5
• UniProt Protein Name: Serine protease inhibitor Kazal-type 5
• Protein Family: Serine protease inhibitor
• UniProt Gene Name: SPINK5
• UniProt Synonym Gene Names: LEKTI
• UniProt Entry Name: ISK5_HUMAN

NCBI Description

This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

SPINK5: Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense- activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin. Defects in SPINK5 are the cause of Netherton syndrome (NETH). NETH is an autosomal recessive congenital ichthyosis associated with hair shaft abnormalities and anomalies of the immune system. Typical features are ichthyosis linearis circumflexa, ichthyosiform erythroderma, trichorrhexis invaginata (bamboo hair), atopic dermatitis, and hayfever. High postnatal mortality is due to failure to thrive, infections and hypernatremic dehydration. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; extracellular region; cell cortex; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: extracellular matrix organization and biogenesis; negative regulation of angiogenesis; epithelial cell differentiation; negative regulation of immune response; epidermal cell differentiation; hair cell differentiation; regulation of T cell differentiation; anagen

Disease: Ige Responsiveness, Atopic; Netherton Syndrome