Rabbit anti-Human LEKTI Polyclonal Antibody | anti-LEKTI antibody

LEKTI (H525) Polyclonal Antibody

Cat. Num. AAA3013737

• Gene Names: SPINK5; NS; NETS; LEKTI; LETKI; VAKTI
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: LEKTI; Polyclonal Antibody; LEKTI (H525) Polyclonal Antibody; Hemofiltrate peptide HF7665; ISK5; Lympho-epithelial Kazal-type-related inhibitor; Lymphoepithelial Kazal type related inhibitor; NETS; NS; Serine peptidase inhibitor Kazal type 5; SPINK5; VAKTI; anti-LEKTI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LEKTI (H525) polyclonal antibody detects endogenous levels of LEKTI protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1094

• Applicable Applications for anti-LEKTI antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 496-543 of Human LEKTI.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of LEKTI (H525) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-LEKTI antibody

Lympho-epithelial Kazal-type inhibitor (LEKTI) is a serine protease inhibitor which protects mucous epithelia against microbial attack and inflammation. LEKTI is a marker of epithelial differentiation and expresses strongly in the granular and uppermost spinous layers of the epidermis and differentiated layers of stratified epithelia. Defects in SPINK5, the gene encoding LEKTI, are the cause of Netherton syndrome, a severe autosomal recessive disorder characterized by atopic dermatitis, hayfever and other conditions.

NCBI and Uniprot Product Information

• NCBI GI #: 189163501
• NCBI GeneID: 11005
• NCBI Accession #: NP_001121170.1
• NCBI GenBank Nucleotide #: NP_001121170.1
• UniProt Accession #: Q9NQ38
• Molecular Weight: ~ 130kDa
• NCBI Official Full Name: serine protease inhibitor Kazal-type 5 isoform a preproprotein
• NCBI Official Synonym Full Names: serine peptidase inhibitor Kazal type 5
• NCBI Official Symbol: SPINK5
• NCBI Official Synonym Symbols: NS; NETS; LEKTI; LETKI; VAKTI
• NCBI Protein Information: serine protease inhibitor Kazal-type 5
• UniProt Protein Name: Serine protease inhibitor Kazal-type 5
• UniProt Gene Name: SPINK5
• UniProt Synonym Gene Names: LEKTI
• UniProt Entry Name: ISK5_HUMAN

NCBI Description

This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

SPINK5: Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense- activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin. Defects in SPINK5 are the cause of Netherton syndrome (NETH). NETH is an autosomal recessive congenital ichthyosis associated with hair shaft abnormalities and anomalies of the immune system. Typical features are ichthyosis linearis circumflexa, ichthyosiform erythroderma, trichorrhexis invaginata (bamboo hair), atopic dermatitis, and hayfever. High postnatal mortality is due to failure to thrive, infections and hypernatremic dehydration. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; extracellular region; cell cortex; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: extracellular matrix organization and biogenesis; negative regulation of angiogenesis; epithelial cell differentiation; negative regulation of immune response; epidermal cell differentiation; hair cell differentiation; regulation of T cell differentiation; anagen

Disease: Ige Responsiveness, Atopic; Netherton Syndrome