Human serine peptidase inhibitor, Kazal type 5 ELISA Kit | SPINK5 elisa kit

Human Serine protease inhibitor Kazal-type 5, SPINK5 ELISA Kit

Cat. Num. AAA942561

• Reactivity: Human
• Synonyms: serine peptidase inhibitor; Kazal type 5; Human Serine protease inhibitor Kazal-type 5; SPINK5 ELISA Kit; Human Serine protease inhibitor Kazal-type 5 (SPINK5) ELISA kit; DKFZp686K19184; FLJ21544; FLJ97536; FLJ97596; FLJ99794; LEKTI; LETKI; NETS; NS; VAKTI; OTTHUMP00000165952; lymphoepithelial Kazal-type-related inhibitor; serine protease inhibitor; SPINK5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human SPINK5. No significant cross-reactivity or interference between human SPINK5 and analogues was observed.
• Sequence Length: 1094

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: < 15.6 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPINK5 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPINK5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPINK5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPINK5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPINK5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 189163501
• NCBI GeneID: 11005
• NCBI Accession #: NP_001121170.1
• NCBI GenBank Nucleotide #: NM_001127698.1
• UniProt Accession #: Q9NQ38; O75770; Q3LX95; Q3LX96; Q3LX97; Q96PP2; Q96PP3; A8MYE8; B7WPB7; D6REN5
• Molecular Weight: 120,714 Da
• NCBI Official Full Name: serine protease inhibitor Kazal-type 5 isoform a
• UniProt Protein Name: Serine protease inhibitor Kazal-type 5
• Protein Family: Serine protease inhibitor
• UniProt Gene Name: SPINK5
• UniProt Synonym Gene Names: LEKTI
• UniProt Entry Name: ISK5_HUMAN

NCBI Description

This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The inhibitor may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SPINK5: Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense- activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin. Defects in SPINK5 are the cause of Netherton syndrome (NETH). NETH is an autosomal recessive congenital ichthyosis associated with hair shaft abnormalities and anomalies of the immune system. Typical features are ichthyosis linearis circumflexa, ichthyosiform erythroderma, trichorrhexis invaginata (bamboo hair), atopic dermatitis, and hayfever. High postnatal mortality is due to failure to thrive, infections and hypernatremic dehydration. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; extracellular region; cell cortex; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: extracellular matrix organization and biogenesis; negative regulation of angiogenesis; epithelial cell differentiation; negative regulation of immune response; hair cell differentiation; epidermal cell differentiation; regulation of T cell differentiation; anagen

Disease: Ige Responsiveness, Atopic; Netherton Syndrome