Human Sperm Adhesion Molecule 1 (SPAM1) ELISA Kit | SPAM1 elisa kit

Human Sperm Adhesion Molecule 1 (SPAM1) ELISA Kit

Cat. Num. AAA2707599

• Gene Names: SPAM1; HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
• Reactivity: Human
• Synonyms: Sperm Adhesion Molecule 1 (SPAM1); Human Sperm Adhesion Molecule 1 (SPAM1) ELISA Kit; HYAL1; HYA1; HYAL3; HYAL5; PH-20; PH20; SPAG15; PH-20 Hyaluronidase; Zona Pellucida Binding; SPAM1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sperm Adhesion Molecule 1 (SPAM1).; No significant cross-reactivity or interference between Sperm Adhesion Molecule 1 (SPAM1) and analogues was observed.
• Sequence Length: 509

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.054ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sperm Adhesion Molecule 1 (SPAM1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sperm Adhesion Molecule 1 (SPAM1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SPAM1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sperm Adhesion Molecule 1 (SPAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sperm Adhesion Molecule 1 (SPAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sperm Adhesion Molecule 1 (SPAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sperm Adhesion Molecule 1 (SPAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 585673
• NCBI GeneID: 6677
• UniProt Accession #: P38567
• NCBI Official Full Name: Hyaluronidase PH-20
• NCBI Official Synonym Full Names: sperm adhesion molecule 1
• NCBI Official Symbol: SPAM1
• NCBI Official Synonym Symbols: HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
• NCBI Protein Information: hyaluronidase PH-20
• UniProt Protein Name: Hyaluronidase PH-20
• Protein Family: Hyaluronidase
• UniProt Gene Name: SPAM1
• UniProt Synonym Gene Names: HYAL3; PH20; Hyal-PH20
• UniProt Entry Name: HYALP_HUMAN

NCBI Description

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

SPAM1: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Belongs to the glycosyl hydrolase 56 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosaminoglycan degradation; Hydrolase; EC 3.2.1.35; Membrane protein, GPI anchor; Cell surface

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: plasma membrane

Molecular Function: hyalurononglucosaminidase activity

Biological Process: sperm-egg recognition; fusion of sperm to egg plasma membrane; binding of sperm to zona pellucida; single fertilization; carbohydrate metabolic process; cell adhesion; multicellular organism reproduction