Testing Data
(Y1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SF-1 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SF-1 (~53kD).)
Rabbit anti-Mouse Steroidogenic Factor 1 Polyclonal Antibody | anti-SF-1 antibody
Steroidogenic Factor 1 (SF-1, NR5A1) (Biotin)
Gene Names
Nr5a1; ELP; SF1; SF-1; Ad4BP; ELP-3; Ftzf1; Ftz-F1
Reactivity
Mouse
Applications
Immunoblot, Western Blot, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Steroidogenic Factor 1; Polyclonal Antibody; Steroidogenic Factor 1 (SF-1; NR5A1) (Biotin); anti-SF-1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse SF-1, Mr 53kD.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-SF-1 antibody
Immunoblot (IB), Western Blot (WB)
Application Notes
IB: 0.5-2ug/ml of this lot detected SF-1 in RIPA lysates from Y1 cells.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
DNA binding domain of SF-1 from mouse, produced in E. coli as a GST fusion protein and partially purified via GST-agarose column chromatography.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Y1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SF-1 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SF-1 (~53kD).)
Testing Data
(Y1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-SF-1 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SF-1 (~53kD).)
Product Categories/Family for anti-SF-1 antibody
References
1. Ikeda, Y., et al., Mol Endocrinol., 7: 852-860, 1993. 2. Lynch, J.P., et al., Mol. Endocrinol., 7: 776-786, 1993. 3. Ingraham, H.A., et al., Genes Dev., 8: 2302-2312, 1994. 4. Hu, Z., et al., J. Biol. Chem., 272: 14263-14271, 1997. 5. Parker, K.L., et al., Recent Prog. Horm. Res., 57: 19-36, 2002. 6. Fowkes, R.C., and Burrin, J.M., J. Endocrinol., 177: 345-350, 2003. 7. Zhao, L., et al., Mol. Cell. Endocrinol., 215: 89-94, 2004.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,077 Da
NCBI Official Full Name
steroidogenic factor 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 5, group A, member 1
NCBI Official Symbol
Nr5a1
NCBI Official Synonym Symbols
ELP; SF1; SF-1; Ad4BP; ELP-3; Ftzf1; Ftz-F1
NCBI Protein Information
steroidogenic factor 1; STF-1; adrenal 4-binding protein; embryonal LTR-binding protein; fushi tarazu 1 factor homolog; fushi tarazu factor homolog 1; steroid hormone receptor Ad4BP; steroid hydroxylase positive regulator; embryonal long terminal repeat-b
UniProt Protein Name
Steroidogenic factor 1
Protein Family
UniProt Gene Name
Nr5a1
UniProt Synonym Gene Names
Ftzf1; SF-1; STF-1; ELP
UniProt Entry Name
STF1_MOUSE
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Product Notes
The SF-1 nr5a1 (Catalog #AAA6501146) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Steroidogenic Factor 1 (SF-1, NR5A1) (Biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Steroidogenic Factor 1 can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB), Western Blot (WB). IB: 0.5-2ug/ml of this lot detected SF-1 in RIPA lysates from Y1 cells. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SF-1 nr5a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Steroidogenic Factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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