Rabbit anti-Human c-Cbl Polyclonal Antibody | anti-c-Cbl antibody

c-Cbl (Ab-700) Antibody

Cat. Num. AAA9403649

• Gene Names: CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: c-Cbl; Polyclonal Antibody; c-Cbl (Ab-700) Antibody; Signal transduction protein CBL; Proto-oncogene c-CBL; Casitas B-lineage lymphoma proto-oncogene; RING finger protein 55; anti-c-Cbl antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total c-Cbl protein.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 906

• Applicable Applications for anti-c-Cbl antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around aa. 698~702 (T-E-Y-M-T) derived from Human c-Cbl.
• Target Name: c-Cbl
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from Hela and HT29 cells using c-Cbl(Ab-700) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Cbl(Ab-700) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Related Product Information for anti-c-Cbl antibody

Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.

Product Categories/Family for anti-c-Cbl antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 52426745
• NCBI GeneID: 867
• NCBI Accession #: NP_005179.2
• NCBI GenBank Nucleotide #: NM_005188.3
• UniProt Accession #: P22681; A3KMP8
• Molecular Weight: 99,633 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CBL
• NCBI Official Synonym Full Names: Cbl proto-oncogene, E3 ubiquitin protein ligase
• NCBI Official Symbol: CBL
• NCBI Official Synonym Symbols: CBL2; NSLL; C-CBL; RNF55; FRA11B
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL
• UniProt Gene Name: CBL
• UniProt Synonym Gene Names: CBL2; RNF55
• UniProt Entry Name: CBL_HUMAN

NCBI Description

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder. [provided by RefSeq, Mar 2012]

Uniprot Description

CBL: an adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. Participates in signal transduction in hematopoietic cells.

Protein type: Adaptor/scaffold; EC 6.3.2.-; Motility/polarity/chemotaxis; Oncoprotein; Ligase; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; plasma membrane; flotillin complex; nucleus; cytosol

Molecular Function: signal transducer activity; protein binding; ephrin receptor binding; zinc ion binding; phosphotyrosine binding; ubiquitin-protein ligase activity; calcium ion binding; transcription factor activity; SH3 domain binding; ligase activity

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase cascade; negative regulation of epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; positive regulation of receptor-mediated endocytosis; negative regulation of apoptosis

Disease: Noonan Syndrome-like Disorder With Or Without Juvenile Myelomonocytic Leukemia