Mouse Sp100 Nuclear Antigen ELISA Kit | Sp100 elisa kit

Mouse Sp100 Nuclear Antigen ELISA Kit

Cat. Num. AAA729479

• Gene Names: SP100; lysp100b
• Reactivity: Mouse
• Synonyms: Sp100 Nuclear Antigen; Mouse Sp100 Nuclear Antigen ELISA Kit; Sp100 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This Sp100 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse Sp100. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Sp100 elisa kit

Principle of the assay: Sp100 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Sp100 antibody and an Sp100-HRP conjugate. The assay sample and buffer are incubated together with Sp100-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Sp100 concentration since Sp100 from samples and Sp100-HRP conjugate compete for the anti-Sp100 antibody binding site. Since the number of sites is limited, as more sites are occupied by Sp100 from the sample, fewer sites are left to bind Sp100-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Sp100 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Sp100 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 1255929
• NCBI GeneID: 6672
• UniProt Accession #: P23497; O75450; Q13343; Q8TE34; Q96F70; Q96T24; Q96T95; B4DDX5; B8ZZD8; E7EUA7; E9PH61; F8WFE2
• Molecular Weight: 50,032 Da
• NCBI Official Full Name: Sp100, partial
• NCBI Official Synonym Full Names: SP100 nuclear antigen
• NCBI Official Symbol: SP100
• NCBI Official Synonym Symbols: lysp100b
• NCBI Protein Information: nuclear autoantigen Sp-100; speckled 100 kDa; SP100-HMG nuclear autoantigen; nuclear dot-associated Sp100 protein
• UniProt Protein Name: Nuclear autoantigen Sp-100
• Protein Family: Nuclear autoantigen
• UniProt Gene Name: SP100
• UniProt Entry Name: SP100_HUMAN

NCBI Description

This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011]

Uniprot Description

SP100: May play a role in the control of gene expression. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nucleoplasm; PML body; Mre11 complex; cytoplasm; nucleolus; nucleus

Molecular Function: protein domain specific binding; identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription coactivator activity; chromatin binding; transcription corepressor activity; transcription factor binding; kinase binding

Biological Process: retinoic acid receptor signaling pathway; negative regulation of protein export from nucleus; response to retinoic acid; viral reproduction; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; negative regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; negative regulation of cell motility; negative regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; negative regulation of DNA binding; chromatin remodeling; negative regulation of viral transcription; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; response to cytokine stimulus; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; telomere maintenance