Human SOS1 (Son of sevenless homolog 1) ELISA Kit | SOS1 elisa kit

Human SOS1 (Son of sevenless homolog 1) ELISA Kit

Cat. Num. AAA7607194

• Gene Names: SOS1; GF1; HGF; NS4; GGF1; GINGF; SOS-1
• Reactivity: Human
• Synonyms: SOS1 (Son of sevenless homolog 1); Human SOS1 (Son of sevenless homolog 1) ELISA Kit; SOS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SOS1. No significant cross-reactivity or interference between SOS1 and analogues was observed.
• Sequence Length: 1333

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOS1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 15529996
• NCBI GeneID: 6654
• NCBI Accession #: NP_005624.2
• NCBI GenBank Nucleotide #: NM_005633.3
• UniProt Accession #: Q07889; A8K2G3; B4DXG2
• Molecular Weight: 36,073 Da
• NCBI Official Full Name: son of sevenless homolog 1
• NCBI Official Synonym Full Names: SOS Ras/Rac guanine nucleotide exchange factor 1
• NCBI Official Symbol: SOS1
• NCBI Official Synonym Symbols: GF1; HGF; NS4; GGF1; GINGF; SOS-1
• NCBI Protein Information: son of sevenless homolog 1
• UniProt Protein Name: Son of sevenless homolog 1
• Protein Family: Son of sevenless
• UniProt Gene Name: SOS1
• UniProt Synonym Gene Names: SOS-1

NCBI Description

This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4. [provided by RefSeq, Jul 2008]

Uniprot Description

Promotes the exchange of Ras-bound GDP by GTP (PubMed:8493579). Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF (PubMed:17339331). Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity ().