NOTCH2 recombinant protein

NOTCH2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120594

• Gene Names: NOTCH2; hN2; AGS2; HJCYS
• Purity: >95% as determined by SDS-PAGE
• Synonyms: NOTCH2; NOTCH2 Protein; Human; Recombinant (His Tag); Human Notch-2 Protein (His Tag); AGS2 Protein; HJCYS Protein; hN2 Protein; notch 2; NOTCH2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10%gly, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln530

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Leu 26
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human NOTCH2 (NP_001186930.1)(Met1-Gln530) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NOTCH2 recombinant protein

Background: NOTCH2 (Notch Receptor 2) is a Protein Coding gene. This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics and play a role in a variety of developmental processes by controlling cell fate decisions. Hajdu Cheney Syndrome (HCS) is a rare disease associated with mutations of NOTCH2 that lead to the translation of a truncated, presumably stable, NOTCH2 protein. NOTCH2 is down-regulated in colon cancer, and reduced expression is associated with a less differentiated, more aggressive phenotype, and reduced overall survival. NOTCH2 has also been shown to have pro-apoptotic and growth-suppressive effects in thyroid carcinoma, and carcinoid tumors. NOTCH2 acts as an oncogene that promotes bladder cancer growth and metastasis through EMT, cell-cycle progression, and maintenance of stemness.

Product Categories/Family for NOTCH2 recombinant protein

Notch Pathway Tumor Suppressors

NCBI and Uniprot Product Information

• NCBI GI #: 317008611
• NCBI GeneID: 4853
• NCBI Accession #: NP_001186930.1
• NCBI GenBank Nucleotide #: NM_001200001.1
• UniProt Accession #: Q04721; Q5T3X7; Q99734; Q9H240
• Molecular Weight: 265,405 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 2 isoform 2
• NCBI Official Synonym Full Names: notch 2
• NCBI Official Symbol: NOTCH2
• NCBI Official Synonym Symbols: hN2; AGS2; HJCYS
• NCBI Protein Information: neurogenic locus notch homolog protein 2; Notch homolog 2
• UniProt Protein Name: Neurogenic locus notch homolog protein 2
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: NOTCH2
• UniProt Synonym Gene Names: Notch 2; hN2
• UniProt Entry Name: NOTC2_HUMAN

NCBI Description

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Notch 2: a member of the Notch family. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. Affects the implementation of differentiation, proliferation and apoptotic programs. Cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for the membrane bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13-p11

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cell surface; membrane; integral to plasma membrane; extracellular region; plasma membrane; nucleus; receptor complex; cilium

Molecular Function: protein binding; receptor activity; calcium ion binding

Biological Process: positive regulation of apoptosis; apoptosis; multicellular organismal development; myeloid dendritic cell differentiation; cell fate determination; negative regulation of transcription from RNA polymerase II promoter; bone remodeling; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; morphogenesis of an epithelial sheet; hemopoiesis; cell cycle arrest; cell growth; positive regulation of BMP signaling pathway; embryonic limb morphogenesis; nervous system development; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; intracellular receptor-mediated signaling pathway; in utero embryonic development; stem cell maintenance; Notch receptor processing; humoral immune response; organ morphogenesis; inflammatory response to antigenic stimulus; defense response to bacterium; gene expression; positive regulation of Ras protein signal transduction; determination of left/right symmetry; negative regulation of apoptosis

Disease: Hajdu-cheney Syndrome; Alagille Syndrome 2