Human Rho-related GTP-binding protein RhoC ELISA Kit | RHOC elisa kit

Human Rho-related GTP-binding protein RhoC ELISA Kit

Cat. Num. AAA764957

• Gene Names: RHOC; H9; ARH9; ARHC; RHOH9
• Reactivity: Human
• Synonyms: Rho-related GTP-binding protein RhoC; Human Rho-related GTP-binding protein RhoC ELISA Kit; RHOC (Rho-related GTP-binding protein RhoC)/ARH9/ARHC/h9/Rho cDNA clone 9; RHOC elisa kit

• Reactivity: Human
• Sequence Length: 193

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for RHOC elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human RHOC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RHOC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RHOC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RHOC can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 111494251
• NCBI GeneID: 389
• NCBI Accession #: NP_001036144.1
• NCBI GenBank Nucleotide #: NM_001042679.1
• Molecular Weight: 22,006 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoC
• NCBI Official Synonym Full Names: ras homolog family member C
• NCBI Official Symbol: RHOC
• NCBI Official Synonym Symbols: H9; ARH9; ARHC; RHOH9
• NCBI Protein Information: rho-related GTP-binding protein RhoC
• UniProt Protein Name: Rho-related GTP-binding protein RhoC
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOC
• UniProt Synonym Gene Names: ARH9; ARHC; h9
• UniProt Entry Name: RHOC_HUMAN

NCBI Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

RHOC: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein, monomeric, Rho; Motility/polarity/chemotaxis; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: cleavage furrow; cytosol; nucleus; plasma membrane

Molecular Function: GTP binding; protein binding; signal transducer activity

Biological Process: apical junction assembly; axon guidance; cytokinesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of lipase activity; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction