Rat ATXN1 ELISA Kit | ATXN1 elisa kit

Rat ATXN1 (Ataxin 1) ELISA Kit

Cat. Num. AAA2509093

• Gene Names: ATXN1; ATX1; SCA1; D6S504E
• Reactivity: Rat
• Synonyms: ATXN1; Rat ATXN1 (Ataxin 1) ELISA Kit; ATXN1 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant Rat ATXN1. No significant cross-reactivity or interference between Rat ATXN1 and analogues was observed.
• Sequence Length: 815

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat ATXN1 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATXN1 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ATXN1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ATXN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ATXN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ATXN1. You can calculate the concentration of ATXN1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 51479158
• NCBI GeneID: 6310
• NCBI Accession #: NP_000323.2
• NCBI GenBank Nucleotide #: NM_000332.3
• UniProt Accession #: P54253; Q17S02; Q9UJG2; Q9Y4J1
• Molecular Weight: 86,923 Da
• NCBI Official Full Name: ataxin-1
• NCBI Official Synonym Full Names: ataxin 1
• NCBI Official Symbol: ATXN1
• NCBI Official Synonym Symbols: ATX1; SCA1; D6S504E
• NCBI Protein Information: ataxin-1; alternative ataxin1; spinocerebellar ataxia type 1 protein
• UniProt Protein Name: Ataxin-1
• Protein Family: Ataxin
• UniProt Gene Name: ATXN1
• UniProt Synonym Gene Names: ATX1; SCA1
• UniProt Entry Name: ATX1_HUMAN

NCBI Description

The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 41-81 CAG repeats, compared to 6-39 in the normal allele, and is associated with spinocerebellar ataxia type 1 (SCA1). At least two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

ataxin-1: spinocerebellar ataxia type 1 (SCA1) protein. Expansion of CAG repeats in the SCA1 gene results in an abnormally long polyglutamine tract in the protein, causing polyglutamine-induced neurodegeneration. 14-3-3 and Akt kinase stabilize both wild-type and mutant forms of ataxin-1. The expansion of the polyglutamine tract may alter this function.

Protein type: Nuclear export; RNA-binding

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: nucleoplasm; nuclear RNA export factor complex; nuclear matrix; intracellular membrane-bound organelle; cytoplasm; nucleus; nuclear inclusion body

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; protein self-association; DNA binding; poly(U) binding; chromatin binding; poly(rG) binding

Biological Process: RNA processing; transcription, DNA-dependent; adult locomotory behavior; nuclear export; negative regulation of insulin-like growth factor receptor signaling pathway; negative regulation of phosphorylation; visual learning; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of excitatory postsynaptic membrane potential; alveolus development

Disease: Spinocerebellar Ataxia 1