Human Sestrin 3 (SESN3) ELISA Kit | SESN3 elisa kit

Human Sestrin 3 (SESN3) ELISA Kit

Cat. Num. AAA2025595

• Gene Names: Sesn3; SEST3; BC003348; 5630400E15Rik
• Reactivity: Human
• Synonyms: Sestrin 3 (SESN3); Human Sestrin 3 (SESN3) ELISA Kit; SEST3; SESN3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sestrin 3 (SESN3).; No significant cross-reactivity or interference between Sestrin 3 (SESN3) and analogues was observed.
• Sequence Length: 492

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sestrin 3 (SESN3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sestrin 3 (SESN3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SESN3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sestrin 3 (SESN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sestrin 3 (SESN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sestrin 3 (SESN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sestrin 3 (SESN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SESN3 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 148693043
• NCBI GeneID: 75747
• Molecular Weight: 57,021 Da
• NCBI Official Full Name: sestrin 3
• NCBI Official Synonym Full Names: sestrin 3
• NCBI Official Symbol: Sesn3
• NCBI Official Synonym Symbols: SEST3; BC003348; 5630400E15Rik
• NCBI Protein Information: sestrin-3
• UniProt Protein Name: Sestrin-3
• Protein Family: Sestrin
• UniProt Gene Name: Sesn3
• UniProt Synonym Gene Names: Sest3
• UniProt Entry Name: SESN3_MOUSE

Uniprot Description

SESN3: a member of the sestrin family of stress-induced proteins. The encoded protein reduces the levels of intracellular reactive oxygen species induced by activated Ras downstream of RAC-alpha serine/threonine-protein kinase (Akt) and FoxO transcription factor. The protein is required for normal regulation of blood glucose, insulin resistance and plays a role in lipid storage in obesity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

Cellular Component: nucleus

Biological Process: regulation of protein kinase B signaling cascade; glucose homeostasis; response to insulin stimulus