Rat Ephrin Type B Receptor 3 (EPHB3) ELISA Kit | EPHB3 elisa kit

Rat Ephrin Type B Receptor 3 (EPHB3) ELISA Kit

Cat. Num. AAA2022936

• Gene Names: EPHB3; ETK2; HEK2; TYRO6
• Reactivity: Rat
• Synonyms: Ephrin Type B Receptor 3 (EPHB3); Rat Ephrin Type B Receptor 3 (EPHB3) ELISA Kit; ETK2; HEK2; TYRO6; EPH-like tyrosine kinase 2; Embryonic kinase 2; Tyrosine-protein kinase TYRO6; EPHB3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ephrin Type B Receptor 3 (EPHB3).; No significant cross-reactivity or interference between Ephrin Type B Receptor 3 (EPHB3) and analogues was observed.
• Sequence Length: 998

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.067ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin Type B Receptor 3 (EPHB3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin Type B Receptor 3 (EPHB3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EPHB3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ephrin Type B Receptor 3 (EPHB3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ephrin Type B Receptor 3 (EPHB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ephrin Type B Receptor 3 (EPHB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ephrin Type B Receptor 3 (EPHB3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for EPHB3 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 17975768
• NCBI GeneID: 2049
• NCBI Accession #: NP_004434.2
• NCBI GenBank Nucleotide #: NM_004443.3
• UniProt Accession #: P54753; Q7Z740
• Molecular Weight: 110,330 Da
• NCBI Official Full Name: ephrin type-B receptor 3
• NCBI Official Synonym Full Names: EPH receptor B3
• NCBI Official Symbol: EPHB3
• NCBI Official Synonym Symbols: ETK2; HEK2; TYRO6
• NCBI Protein Information: ephrin type-B receptor 3
• UniProt Protein Name: Ephrin type-B receptor 3
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB3
• UniProt Synonym Gene Names: ETK2; HEK2; TYRO6; EPH-like kinase 2; EK2; hEK2
• UniProt Entry Name: EPHB3_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members. [provided by RefSeq, Mar 2010]

Uniprot Description

EphB3: a ubiquitous receptor tyrosine kinase of the Eph family. A receptor for ephrin-B family members: ephrin-B1 and -B2. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels.EphB3 contains 1 sterile alpha motif (SAM) domain and 2 fibronectin type III domains.

Protein type: Protein kinase, tyrosine (receptor); EC 2.7.10.1; Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; Eph family

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: integral to plasma membrane; dendrite; plasma membrane; extracellular region; cytosol

Molecular Function: ephrin receptor activity; axon guidance receptor activity; ATP binding

Biological Process: axon guidance; cell migration; peptidyl-tyrosine phosphorylation; thymus development; protein amino acid autophosphorylation; axonal fasciculation; central nervous system projection neuron axonogenesis; digestive tract morphogenesis; palate development; regulation of axonogenesis; corpus callosum development; positive regulation of synaptogenesis; regulation of cell-cell adhesion; ephrin receptor signaling pathway; angiogenesis; urogenital system development; retinal ganglion cell axon guidance