Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein kinase RIO1 (RIOK1) Standard Curve.)
Human Serine/Threonine-Protein Kinase RIO1 (RIOK1) ELISA Kit | RIOK1 elisa kit
Human Serine/Threonine-Protein Kinase RIO1 (RIOK1) ELISA Kit
Gene Names
RIOK1; RIO1; AD034; RRP10; bA288G3.1
Reactivity
Human
Synonyms
Serine/Threonine-Protein Kinase RIO1 (RIOK1); Human Serine/Threonine-Protein Kinase RIO1 (RIOK1) ELISA Kit; RIOK1; AD034; FLJ30006; MGC12903; bA288G3.1; RIO kinase 1; prematurely truncated due to insertion of intronic sequence; RIOK1 elisa kit
Reactivity
Human
Specificity
Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RIOK1. No significant cross-reactivity or interference between Human RIOK1 and analogues was observed.
Sequence Length
568
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein kinase RIO1 (RIOK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein kinase RIO1 (RIOK1) Standard Curve.)
Related Product Information for RIOK1 elisa kit
Description: This Human Serine/threonine-protein kinase RIO1 (RIOK1) ELISA Kit employs a two-site sandwich ELISA to quantitate RIOK1 in samples. An antibody specific for RIOK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RIOK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RIOK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RIOK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RIOK1 includes two alternatively spliced transcript variants, which encode different isoforms. The function of this gene has not been determined. RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. RioK1 and pICln bind to PRMT5 in a mutually exclusive fashion. This results in a PRMT5-WD45/MEP50 core structure that either associates with pICln or RioK1 in distinct complexes. RioK1 functions in analogy to pICln as an adapter protein by recruiting the RNA-binding protein nucleolin to the PRMT5 complex for its symmetrical methylation. The exclusive interaction of PRMT5 with either pICln or RioK1 thus provides the first mechanistic insight into how a methyltransferase can distinguish between its substrate proteins.
Background: RIOK1 includes two alternatively spliced transcript variants, which encode different isoforms. The function of this gene has not been determined. RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. RioK1 and pICln bind to PRMT5 in a mutually exclusive fashion. This results in a PRMT5-WD45/MEP50 core structure that either associates with pICln or RioK1 in distinct complexes. RioK1 functions in analogy to pICln as an adapter protein by recruiting the RNA-binding protein nucleolin to the PRMT5 complex for its symmetrical methylation. The exclusive interaction of PRMT5 with either pICln or RioK1 thus provides the first mechanistic insight into how a methyltransferase can distinguish between its substrate proteins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine/threonine-protein kinase RIO1 isoform 1
NCBI Official Synonym Full Names
RIO kinase 1
NCBI Official Symbol
RIOK1
NCBI Official Synonym Symbols
RIO1; AD034; RRP10; bA288G3.1
NCBI Protein Information
serine/threonine-protein kinase RIO1
UniProt Protein Name
Serine/threonine-protein kinase RIO1
UniProt Gene Name
RIOK1
NCBI Description
The protein encoded by this gene competes with pICln for inclusion in the protein arginine methyltransferase 5 complex. This complex targets substrates for dimethylation. The encoded protein is essential for the last steps in the maturation of 40S subunits. [provided by RefSeq, Jan 2017]
Research Articles on RIOK1
Similar Products
Product Notes
The Human RIOK1 riok1 (Catalog #AAA9714460) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714460 ELISA Kit recognizes Human RIOK1. It is sometimes possible for the material contained within the vial of "Serine/Threonine-Protein Kinase RIO1 (RIOK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
