Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Semaphorin-4A (SEMA4A) Standard Curve.)
Human Semaphorin-4A (SEMA4A) ELISA Kit | SEMA4A elisa kit
Human Semaphorin-4A (SEMA4A) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Semaphorin-4A (SEMA4A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Semaphorin-4A (SEMA4A) Standard Curve.)
Background: SEMA4A is a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in guidance of axonal migration during neuronal development and in immune responses.Mice treated with Sema4a had enhanced priming of antigen-specific T cells secreting both IL4 and gamma-interferon (IFNG). Treatment of mice with anti-Sema4a early after immunization with myelin oligodendrocyte glycoprotein suppressed the development of experimental autoimmune encephalomyelitis. In these mice, infiltration of mononuclear inflammatory cells was ablated by inhibition of the generation of antigen-specific T cells by anti-Sema4a. Expression cloning determined that the high-affinity receptor for Sema4a is Tim2. Sema4a stimulation of cells expressing Tim2 induced tyrosine phosphorylation of Tim2 but not of Tim3.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]
Uniprot Description
SEMA4A: a single-pass type I membrane protein that inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Defects in SEMA4A are the cause of retinitis pigmentosa type 35 and cone-rod dystrophy type 10.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: integral to membrane; plasma membrane
Molecular Function: protein binding; receptor activity
Biological Process: regulation of cell shape; axon guidance; negative regulation of angiogenesis; T-helper 1 cell differentiation; angiogenesis
Disease: Retinitis Pigmentosa 35; Cone-rod Dystrophy 10
Research Articles on SEMA4A
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Product Notes
The Human SEMA4A sema4a (Catalog #AAA9714378) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714378 ELISA Kit recognizes Human SEMA4A. It is sometimes possible for the material contained within the vial of "Semaphorin-4A (SEMA4A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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