Rat Neurotrophic Tyrosine Kinase Receptor Type 2 ELISA Kit | NTRK2 elisa kit

Rat Neurotrophic Tyrosine Kinase Receptor Type 2 ELISA Kit

Cat. Num. AAA762067

• Gene Names: NTRK2; TRKB; trk-B; GP145-TrkB
• Reactivity: Rat
• Synonyms: Neurotrophic Tyrosine Kinase Receptor Type 2; Rat Neurotrophic Tyrosine Kinase Receptor Type 2 ELISA Kit; NTRK2/BDNF; NT-3 growth factors receptor/GP145-TrkB/neurotrophic tyrosine kinase; receptor; type 2/trk-B/Trk-B/TrkB tyrosine kinase/TRKBBDNF-tropomyosine receptor kinase B/tyrosine kinase receptor B; NTRK2 elisa kit

• Reactivity: Rat
• Sequence Length: 822

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 93.75-6000pg/ml
• Sensitivity: 56.25pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NTRK2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat NTRK2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat NTRK2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat NTRK2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat NTRK2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 15217077
• NCBI GeneID: 4915
• Molecular Weight: 35,332 Da
• NCBI Official Full Name: neurotrophic tyrosine kinase receptor type 2
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 2
• NCBI Official Symbol: NTRK2
• NCBI Official Synonym Symbols: TRKB; trk-B; GP145-TrkB
• NCBI Protein Information: BDNF/NT-3 growth factors receptor
• UniProt Protein Name: BDNF/NT-3 growth factors receptor
• Protein Family: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: NTRK2
• UniProt Synonym Gene Names: TRKB; Trk-B
• UniProt Entry Name: NTRK2_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described.

Protein type: Membrane protein, integral; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Trk family

Chromosomal Location of Human Ortholog: 9q22.1

Cellular Component: cell surface; cytosol; dendritic spine; endosome membrane; growth cone; integral to plasma membrane; perikaryon; postsynaptic membrane; presynaptic active zone; receptor complex; rough endoplasmic reticulum; terminal button

Molecular Function: ATP binding; brain-derived neurotrophic factor binding; brain-derived neurotrophic factor receptor activity; neurotrophin binding; protein homodimerization activity; receptor tyrosine kinase binding

Biological Process: adenylate cyclase activation; aging; brain-derived neurotrophic factor receptor signaling pathway; central nervous system neuron development; cerebral cortex development; circadian rhythm; feeding behavior; glutamate secretion; inflammatory response; learning; long-term memory; mechanoreceptor differentiation; negative regulation of neuron apoptosis; nerve growth factor receptor signaling pathway; neuromuscular junction development; neuron differentiation; neuron migration; oligodendrocyte differentiation; peptidyl-tyrosine phosphorylation; peripheral nervous system neuron development; positive regulation of axonogenesis; positive regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; protein amino acid autophosphorylation; regulation of dendrite development; regulation of GTPase activity; regulation of neurotransmitter secretion; regulation of protein kinase B signaling cascade; response to light stimulus; retina development in camera-type eye; retinal rod cell development; transmembrane receptor protein tyrosine kinase signaling pathway; vasculogenesis

Disease: Obesity, Hyperphagia, And Developmental Delay