Human Semaphorin 4A (SEMA4A) ELISA Kit | SEMA4A elisa kit

Human Semaphorin 4A (SEMA4A) ELISA Kit

Cat. Num. AAA454356

• Gene Names: SEMA4A; RP35; SEMB; SEMAB; CORD10
• Reactivity: Human
• Synonyms: Semaphorin 4A (SEMA4A); Human Semaphorin 4A (SEMA4A) ELISA Kit; SEMA4A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SEMA4A. No significant cross-reactivity or interference between SEMA4A and analogues was observed.
• Sequence Length: 761

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.116ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SEMA4A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SEMA4A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA4A elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA4A in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEMA4A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SEMA4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SEMA4A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 300863072
• NCBI GeneID: 64218
• NCBI Accession #: NP_001180229.1
• NCBI GenBank Nucleotide #: NM_001193300.1
• UniProt Accession #: Q9H3S1; Q5TCI5; Q5TCJ6; Q8WUA9; B2RDH8; B3KR76
• Molecular Weight: 69,104 Da
• NCBI Official Full Name: semaphorin-4A isoform 1
• NCBI Official Synonym Full Names: semaphorin 4A
• NCBI Official Symbol: SEMA4A
• NCBI Official Synonym Symbols: RP35; SEMB; SEMAB; CORD10
• NCBI Protein Information: semaphorin-4A
• UniProt Protein Name: Semaphorin-4A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA4A
• UniProt Synonym Gene Names: SEMAB; SEMB; Sema B

NCBI Description

This gene encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]

Uniprot Description

Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. Plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. Promotes phosphorylation of TIMD2. Inhibits angiogenesis. Promotes axon growth cone collapse. Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons ().