Mouse Semaphorin 3F (SEMA3F) ELISA Kit | SEMA3F elisa kit

Mouse Semaphorin 3F (SEMA3F) ELISA Kit

Cat. Num. AAA2707015

• Gene Names: SEMA3F; SEMA4; SEMAK; SEMA-IV
• Reactivity: Mouse
• Synonyms: Semaphorin 3F (SEMA3F); Mouse Semaphorin 3F (SEMA3F) ELISA Kit; SEMA-IV; SEMA4; SEMAK; Sema Domain; Immunoglobulin Domain(Ig); Transmembrane Domain(TM)and Short Cytoplasmic Domain 3F; SEMA3F elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 3F (SEMA3F).; No significant cross-reactivity or interference between Semaphorin 3F (SEMA3F) and analogues was observed.
• Sequence Length: 785

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 3F (SEMA3F) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 3F (SEMA3F) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA3F elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3F (SEMA3F). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 3F (SEMA3F). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 3F (SEMA3F), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 3F (SEMA3F) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 188497703
• NCBI GeneID: 6405
• NCBI Accession #: NP_004177.3
• NCBI GenBank Nucleotide #: NM_004186.4
• UniProt Accession #: Q13275; Q13274; Q13372; Q15704; Q6GTR4; C9JQ85
• Molecular Weight: 85,064 Da
• NCBI Official Full Name: semaphorin-3F isoform 1
• NCBI Official Synonym Full Names: semaphorin 3F
• NCBI Official Symbol: SEMA3F
• NCBI Official Synonym Symbols: SEMA4; SEMAK; SEMA-IV
• NCBI Protein Information: semaphorin-3F
• UniProt Protein Name: Semaphorin-3F
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA3F
• UniProt Synonym Gene Names: Sema IV

NCBI Description

This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin loop and a C-terminal basic domain. This gene is expressed by the endothelial cells where it was found to act in an autocrine fashion to induce apoptosis, inhibit cell proliferation and survival, and function as an anti-tumorigenic agent. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

SEMA3F: May play a role in cell motility and cell adhesion. Belongs to the semaphorin family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular space

Molecular Function: chemorepellent activity

Biological Process: axon extension involved in axon guidance; axon guidance; branchiomotor neuron axon guidance; facial nerve structural organization; negative chemotaxis; negative regulation of axon extension involved in axon guidance; nerve development; trigeminal nerve structural organization