Human ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) RTU ELISA Kit | ATP1b4 rtu elisa kit

Human ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) ELISA Kit

Cat. Num. AAA457820

• Reactivity: Human
• Synonyms: ATPase; Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4); Human ATPase; Na+/K+ Transporting Beta 4 Polypeptide (ATP1b4) ELISA Kit; X; K-ATPase subunit beta-m; X/potassium-transporting ATPase subunit beta-m; ATP1b4 rtu elisa kit

• Reactivity: Human
• Sequence Length: 357

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.106ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP1b4 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP1b4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ATP1b4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP1b4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP1b4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 215490093
• NCBI GeneID: 23439
• NCBI Accession #: NP_001135919.1
• NCBI GenBank Nucleotide #: NM_001142447.2
• UniProt Accession #: Q9UN42; Q17RR0; Q9UN41
• Molecular Weight: 41,109 Da
• NCBI Official Full Name: protein ATP1B4 isoform A
• NCBI Official Synonym Full Names: ATPase Na+/K+ transporting family member beta 4
• NCBI Official Symbol: ATP1B4
• NCBI Protein Information: protein ATP1B4
• UniProt Protein Name: Protein ATP1B4
• Protein Family: Protein
• UniProt Gene Name: ATP1B4

NCBI Description

This gene has been found in all vertebrate genomes sequenced to date. However, this gene has undergone a change in function in placental mammals compared to other species. Specifically, in fish, avian, and amphibian species, this gene encodes plasma membrane-bound beta-subunits of Na,K-ATPase. In placental mammals, the encoded protein interacts with the nuclear transcriptional coregulator SKIP and may be involved in the regulation of TGF-beta signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

May act as a transcriptional coregulator during muscle development through its interaction with SNW1. Has lost its ancestral function as a Na,K-ATPase beta-subunit.