Human SCGB1A1/uteroglobin ELISA Kit | SCGB1A1 elisa kit

Human SCGB1A1/uteroglobin PicoKine ELISA Kit

Cat. Num. AAA176550

• Gene Names: SCGB1A1; UGB; UP1; CC10; CC16; CCSP; UP-1; CCPBP
• Reactivity: Human
• Synonyms: SCGB1A1/uteroglobin; Human SCGB1A1/uteroglobin PicoKine ELISA Kit; Uteroglobin; secretoglobin; family 1A; member 1 (uteroglobin); blastokinin; CC10; CC16; CCPBP; CCSP; Clara cell phospholipid binding protein; Clara cell phospholipid-binding protein; clara cell secretory protein; Clara cell specific 10 kD protein; Clara Cells 10 kDa Secretory Protein; OTTHUMP00000236107; SCGB1A1; Secretoglobin family 1A member 1; Secretoglobin; UG; UGB; UP-1; UP1; Urinary Protein 1; Urine Protein 1; UTER_HUMAN; Utg; SCGB1A1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human SCGB1A1
• Sequence Length: 91

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human SCGB1A1/uteroglobin PicoKine ELISA Kit standard curve )

Related Product Information for SCGB1A1 elisa kit

Principle of the assay: human SCGB1A1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SCGB1A1 has been precoated onto 96-well plates. Standards(E.coli, E22-N91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SCGB1A1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SCGB1A1 amount of sample captured in plate.
Background: SCGB1A1(secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A(2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.

NCBI and Uniprot Product Information

• NCBI GI #: 112672
• NCBI GeneID: 7356
• UniProt Accession #: P11684; Q6FHH3; Q9UCM2; Q9UCM4; B2R5F2
• Molecular Weight: 9,994 Da
• NCBI Official Full Name: Uteroglobin
• NCBI Official Synonym Full Names: secretoglobin, family 1A, member 1 (uteroglobin)
• NCBI Official Symbol: SCGB1A1
• NCBI Official Synonym Symbols: UGB; UP1; CC10; CC16; CCSP; UP-1; CCPBP
• NCBI Protein Information: uteroglobin; blastokinin; urine protein 1; urinary protein 1; clara cells 10 kDa secretory protein; clara cell phospholipid-binding protein
• UniProt Protein Name: Uteroglobin
• Protein Family: Uteroglobin
• UniProt Gene Name: SCGB1A1
• UniProt Synonym Gene Names: CC10; CCSP; UGB; CCPBP; CC10; UP-1; UP1; Urine protein 1
• UniProt Entry Name: UTER_HUMAN

NCBI Description

This gene encodes a member of the secretoglobin family of small secreted proteins. The encoded protein has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma. [provided by RefSeq, May 2010]

Uniprot Description

SCGB1A1: Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2. Belongs to the secretoglobin family.

Protein type: Cell cycle regulation; Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: extracellular space; rough endoplasmic reticulum; nuclear envelope; secretory granule

Molecular Function: protein binding; phospholipase A2 inhibitor activity

Biological Process: response to drug; negative regulation of interleukin-4 production; response to glucocorticoid stimulus; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; female pregnancy; signal transduction; regulation of mRNA stability; response to xenobiotic stimulus; negative regulation of interleukin-13 production; response to silicon dioxide; negative regulation of T cell proliferation; response to ozone; response to cytokine stimulus; negative regulation of interferon-gamma production; regulation of inflammatory response; negative regulation of catalytic activity; negative regulation of interleukin-5 production; embryo implantation

Disease: Asthma, Susceptibility To