Monkey Protein Kinase C Theta ELISA Kit | PKCq elisa kit

Monkey Protein Kinase C Theta ELISA Kit

Cat. Num. AAA745630

• Gene Names: PRKCQ; PRKCT; nPKC-theta
• Reactivity: Monkey
• Synonyms: Protein Kinase C Theta; Monkey Protein Kinase C Theta ELISA Kit; PKCq elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PKCq ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey PKCq. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKCq elisa kit

Principle of the assay: PKCq ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKCq antibody and an PKCq-HRP conjugate. The assay sample and buffer are incubated together with PKCq-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKCq concentration since PKCq from samples and PKCq-HRP conjugate compete for the anti-PKCq antibody binding site. Since the number of sites is limited, as more sites are occupied by PKCq from the sample, fewer sites are left to bind PKCq-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKCq concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PKCq elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 5453976
• NCBI GeneID: 5588
• NCBI Accession #: NP_006248.1
• NCBI GenBank Nucleotide #: NM_006257.4
• Molecular Weight: 67,560 Da
• NCBI Official Full Name: protein kinase C theta type isoform 1
• NCBI Official Synonym Full Names: protein kinase C, theta
• NCBI Official Symbol: PRKCQ
• NCBI Official Synonym Symbols: PRKCT; nPKC-theta
• NCBI Protein Information: protein kinase C theta type
• UniProt Protein Name: Protein kinase C theta type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCQ
• UniProt Synonym Gene Names: PRKCT
• UniProt Entry Name: KPCT_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCT: an AGC kinase of the PKC family. A novel PKC: Ca2+ independent but still regulated by PS, DAG and phorbol esters. Contains 2 zinc-dependent phorbol-ester and DAG binding domains. Expressed in skeletal muscle and hematopoietic cells. May play a role in T cell receptor signaling and insulin resistance. Required for TCR-induced NF-kappaB activation in mature T lymphocytes.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; Kinase, protein; AGC group; PKC family; Delta subfamily

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: plasma membrane; immunological synapse; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ubiquitin-protein ligase activity; ATP binding

Biological Process: positive regulation of interleukin-17 production; platelet activation; phototransduction, visible light; axon guidance; apoptosis; positive regulation of interleukin-2 biosynthetic process; membrane protein ectodomain proteolysis; protein ubiquitination; negative regulation of insulin receptor signaling pathway; protein amino acid phosphorylation; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; positive regulation of interleukin-4 production; rhodopsin mediated signaling; regulation of rhodopsin mediated signaling; regulation of transcription, DNA-dependent; innate immune response; positive regulation of T cell proliferation; regulation of cell growth; positive regulation of T cell activation; inflammatory response; blood coagulation; cell structure disassembly during apoptosis