Human S100 Calcium Binding Protein A2 ELISA Kit | S100A2 elisa kit

Human S100 Calcium Binding Protein A2 ELISA Kit

Cat. Num. AAA7254505

• Gene Names: S100A2; CAN19; S100L
• Reactivity: Human
• Synonyms: S100 Calcium Binding Protein A2; Human S100 Calcium Binding Protein A2 ELISA Kit; S100A2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of S100A2. No significant cross-reactivity or interference between S100A2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S100A2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Sequence Length: 98

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/ml

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for S100A2 elisa kit

Intended Uses: This S100A2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human S100A2. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: S100A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-S100A2 antibody and an S100A2-HRP conjugate. The assay sample and buffer are incubated together with S100A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100A2 concentration since S100A2 from samples and S100A2-HRP conjugate compete for the anti-S100A2 antibody binding site. Since the number of sites is limited, as more sites are occupied by S100A2 from the sample, fewer sites are left to bind S100A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119573690
• NCBI GeneID: 6273
• Molecular Weight: 11,117 Da
• NCBI Official Full Name: S100 calcium binding protein A2
• NCBI Official Synonym Full Names: S100 calcium binding protein A2
• NCBI Official Symbol: S100A2
• NCBI Official Synonym Symbols: CAN19; S100L
• NCBI Protein Information: protein S100-A2
• UniProt Protein Name: Protein S100-A2
• Protein Family: Protein
• UniProt Gene Name: S100A2
• UniProt Synonym Gene Names: S100L

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth.