Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra) Polyclonal Antibody | anti-CSF2Ra antibody
Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra)
Gene Names
Csf2ra; CD116; Csfgmra; GM-CSFR; GM-CSF-Ra
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra); Polyclonal Antibody; Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra); anti-CSF2Ra antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CSF2Ra. It has been selected for its ability to recognize CSF2Ra in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-LDVNGTVG GAAAHWRLSF VNEGAAGSGA ENLTCEIRAA RFLSCAWREG PAAPADVRYS LRVLNSTGHD VARCMADPGD DVITQCIAND LSLLGSEAYL VVTGRSGAGP VRFLDDVVAT KALERLGPPR DVTASCNSSH CTVSWAPPST WASLTARDFQ FEVQ
MGHHHHHHSGSEF-LDVNGTVG GAAAHWRLSF VNEGAAGSGA ENLTCEIRAA RFLSCAWREG PAAPADVRYS LRVLNSTGHD VARCMADPGD DVITQCIAND LSLLGSEAYL VVTGRSGAGP VRFLDDVVAT KALERLGPPR DVTASCNSSH CTVSWAPPST WASLTARDFQ FEVQ
Sequence Length
434
Applicable Applications for anti-CSF2Ra antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CSF2Ra (Leu103~Gln264) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2055826
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,769 Da
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor receptor subunit alpha
NCBI Official Synonym Full Names
colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
NCBI Official Symbol
Csf2ra
NCBI Official Synonym Symbols
CD116; Csfgmra; GM-CSFR; GM-CSF-Ra
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor receptor subunit alpha
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor receptor subunit alpha
Protein Family
UniProt Gene Name
Csf2ra
UniProt Synonym Gene Names
Csfgmra; GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha
Uniprot Description
CSF2RA: Low affinity receptor for granulocyte-macrophage colony- stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. Defects in CSF2RA are the cause of pulmonary surfactant metabolism dysfunction type 4 (SMDP4). A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Belongs to the type I cytokine receptor family. Type 5 subfamily. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 19 D3|19 56.89 cM
Cellular Component: integral component of membrane; membrane; plasma membrane
Molecular Function: granulocyte colony-stimulating factor binding; granulocyte colony-stimulating factor receptor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor activity
Disease: Surfactant Metabolism Dysfunction, Pulmonary, 4
Research Articles on CSF2Ra
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Product Notes
The CSF2Ra csf2ra (Catalog #AAA2001666) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CSF2Ra csf2ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-LDVNGT VG GAAAHWRLSF VNEGAAGSGA ENLTCEIRAA RFLSCAWREG PAAPADVRYS LRVLNSTGHD VARCMADPGD DVITQCIAND LSLLGSEAYL VVTGRSGAGP VRFLDDVVAT KALERLGPPR DVTASCNSSH CTVSWAPPST WASLTARDFQ FEVQ. It is sometimes possible for the material contained within the vial of "Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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