Rabbit anti-Human, Rat TAF II Polyclonal Antibody | anti-TAFII antibody

TAF II p100 (G411) Polyclonal Antibody

Cat. Num. AAA3002686

• Gene Names: TAF5; TAF2D; TAFII100; TAFII-100; TAF(II)100
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TAF II; Polyclonal Antibody; TAF II p100 (G411) Polyclonal Antibody; Transcription initiation factor TFIID subunit 5; Transcription initiation factor TFIID 100 kDa subunit; TAF(II)100; TAFII-100; TAFII100; TAF5; TAF2D; anti-TAFII antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TAF II p100 (G411) polyclonal antibody detects endogenous levels of TAF II p100 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 800

• Applicable Applications for anti-TAFII antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 380-432 of Human TAF II p100.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TAF II p100 (G411) pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:The Lung tissue lysate of Rat (20ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TAF II p100 (G411) pAb in paraffin-embedded liver cancer tissue at 1:100.)

Related Product Information for anti-TAFII antibody

TFIID is a general transcription factor which initiates preinitiation complex assembly through direct interaction with the TATA promoter element. It is a multisubunit complex consisting of a small TATA-binding polypeptide andother TATA-binding protein (TBP)-associated factors (TAFs). Although native TFIID can mediate both activator-independent (basal) and activator-dependent transcription in reconstituted systems, TBP can mediate only basal transcription. TAF II p100 (TBP-associated factor II100), also known as TAF5 or TAFII100, is the third largest subunit of human TFIID. It contains six WD40 repeats at the C-terminus and has an N-terminus capable of forming a flexible dimer. TAF II p100 plays an important role in forming the scaffold that is crucial for the assembly of the TFIID complex. TAF II p100 may also be involved in the stabilization of TAF interactions.

NCBI and Uniprot Product Information

• NCBI GI #: 21071067
• NCBI GeneID: 6877
• NCBI Accession #: NP_008882.2
• NCBI GenBank Nucleotide #: NP_008882.2
• UniProt Accession #: Q15542
• Molecular Weight: ~ 100kDa
• NCBI Official Full Name: transcription initiation factor TFIID subunit 5 isoform 1
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor 5
• NCBI Official Symbol: TAF5
• NCBI Official Synonym Symbols: TAF2D; TAFII100; TAFII-100; TAF(II)100
• NCBI Protein Information: transcription initiation factor TFIID subunit 5
• UniProt Protein Name: Transcription initiation factor TFIID subunit 5
• UniProt Gene Name: TAF5
• UniProt Synonym Gene Names: TAF2D; TAF(II)100; TAFII-100; TAFII100
• UniProt Entry Name: TAF5_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

TAF5: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed. Belongs to the WD repeat TAF5 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 10q24-q25.2

Cellular Component: nucleoplasm; transcription factor TFIID complex; intracellular membrane-bound organelle; nucleolus; nucleus; actin cytoskeleton

Molecular Function: protein dimerization activity; protein binding; histone acetyltransferase activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; viral reproduction; RNA elongation from RNA polymerase II promoter; transcription initiation; gene expression; chromatin modification; histone acetylation