Western Blot (WB)
(Western Blot detection against Immunogen (35.64kD).)
Mouse anti-Human RAPGEF2 Monoclonal Antibody | anti-RAPGEF2 antibody
RAPGEF2 (Rap Guanine Nucleotide Exchange Factor 2, Neural RAP Guanine Nucleotide Exchange Protein, nRap GEP, PDZ Domain-containing Guanine Nucleotide Exchange Factor 1, PDZ-GEF1, RA-GEF, KIAA0313, NRAPGEP, PDZGEF1) (Biotin)
Gene Names
RAPGEF2; RAGEF; RA-GEF; NRAPGEP; PDZGEF1; Rap-GEP; CNrasGEF; PDZ-GEF1; RA-GEF-1; nRap GEP
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RAPGEF2; Monoclonal Antibody; RAPGEF2 (Rap Guanine Nucleotide Exchange Factor 2; Neural RAP Guanine Nucleotide Exchange Protein; nRap GEP; PDZ Domain-containing Guanine Nucleotide Exchange Factor 1; PDZ-GEF1; RA-GEF; KIAA0313; NRAPGEP; PDZGEF1) (Biotin); anti-RAPGEF2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1E8
Specificity
Recognizes human RAPGEF2.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-RAPGEF2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa1398-1487 from RAPGEF2 (XP_376350) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
PITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (35.64kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (35.64kD).)
Testing Data
(Detection limit for recombinant GST tagged RAPGEF2 is ~0.1ng/ml as a capture antibody)
Testing Data
(Detection limit for recombinant GST tagged RAPGEF2 is ~0.1ng/ml as a capture antibody)
Related Product Information for anti-RAPGEF2 antibody
Guanine nucleotide exchange factor (GEF) for Rap1A, Rap1B and Rap2B GTPases. Does not interact with cAMP or cGMP.
Product Categories/Family for anti-RAPGEF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor 2
NCBI Official Symbol
RAPGEF2
NCBI Official Synonym Symbols
RAGEF; RA-GEF; NRAPGEP; PDZGEF1; Rap-GEP; CNrasGEF; PDZ-GEF1; RA-GEF-1; nRap GEP
NCBI Protein Information
rap guanine nucleotide exchange factor 2
Protein Family
NCBI Description
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]
Research Articles on RAPGEF2
Similar Products
Product Notes
The RAPGEF2 (Catalog #AAA6143920) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAPGEF2 (Rap Guanine Nucleotide Exchange Factor 2, Neural RAP Guanine Nucleotide Exchange Protein, nRap GEP, PDZ Domain-containing Guanine Nucleotide Exchange Factor 1, PDZ-GEF1, RA-GEF, KIAA0313, NRAPGEP, PDZGEF1) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAPGEF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RAPGEF2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAPGEF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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