Human S100 Calcium Binding Protein A1 (S100A1) ELISA Kit | S100A1 elisa kit

Human S100 Calcium Binding Protein A1 (S100A1) ELISA Kit

Cat. Num. AAA1600007

• Gene Names: S100A1; S100; S100A; S100-alpha
• Reactivity: Human
• Synonyms: S100 Calcium Binding Protein A1 (S100A1); Human S100 Calcium Binding Protein A1 (S100A1) ELISA Kit; S100A1 elisa kit

• Reactivity: Human
• Sequence Length: 94

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 5ng/L - 2000ng/L
• Sensitivity: 3.21ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A1 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human S100 Calcium Binding Protein A1 (also known as S100A1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human S100A1 antibody. S100A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human S100A1 Antibody is added and binds to S100A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated S100A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human S100A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 957950152
• NCBI GeneID: 6271
• Molecular Weight: 10,546 Da
• NCBI Official Full Name: S100 calcium binding protein A1 isoform 1, partial
• NCBI Official Synonym Full Names: S100 calcium binding protein A1
• NCBI Official Symbol: S100A1
• NCBI Official Synonym Symbols: S100; S100A; S100-alpha
• NCBI Protein Information: protein S100-A1
• UniProt Protein Name: Protein S100-A1
• Protein Family: Protein
• UniProt Gene Name: S100A1
• UniProt Synonym Gene Names: S100A

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008]

Uniprot Description

Probably acts as a Ca2+ signal transducer (PubMed:22399290). In response to an increase in intracellular Ca2+ levels, binds calcium which triggers a conformational change (PubMed:23351007). This conformational change allows interaction of S1001A with specific target proteins, such as TPR-containing proteins, and the modulation of their activity (PubMed:22399290).