Rabbit anti-Human EDD Polyclonal Antibody | anti-EDD antibody

EDD Antibody

Cat. Num. AAA9403344

• Gene Names: UBR5; DD5; EDD; HYD; EDD1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: EDD; Polyclonal Antibody; EDD Antibody; CAK; CD167a antigen; Cell adhesion kinase; Discoidin receptor tyrosine kinase; EC 2.7.1.112; anti-EDD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total EDD protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 2798

• Applicable Applications for anti-EDD antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from Internal of human EDD.
• Target Name: EDD
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using EDD antibody.)

Western Blot (WB)

(Western blot analysis of extracts from A549 cells, using EDD antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using EDD antiobdy. The lane on the left is treated with systhesized peptide.)

Related Product Information for anti-EDD antibody

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation By similarity. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.Michelle J. Henderson, J. Biol. Chem., Dec 2006; 281: 39990 - 40000. Lisa N. Kinch, Protein Sci., Feb 2005; 14: 360 - 367. Michelle J. Henderson, J. Biol. Chem., Jul 2002; 277: 26468 - 26478. Darren N. Saunders, Mol. Cell. Biol., Aug 2004; 24: 7225 - 7

Product Categories/Family for anti-EDD antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 544583486
• NCBI GeneID: 51366
• NCBI Accession #: NP_001269802.1
• NCBI GenBank Nucleotide #: NM_001282873.1
• UniProt Accession #: O95071; O94970; Q9NPL3; B2RP24; J3KMW7
• Molecular Weight: 309,224 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase UBR5 isoform 2
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3 component n-recognin 5
• NCBI Official Symbol: UBR5
• NCBI Official Synonym Symbols: DD5; EDD; HYD; EDD1
• NCBI Protein Information: E3 ubiquitin-protein ligase UBR5
• UniProt Protein Name: E3 ubiquitin-protein ligase UBR5
• Protein Family: Phosphogluconate dehydratase
• UniProt Gene Name: UBR5
• UniProt Synonym Gene Names: EDD; EDD1; HYD; KIAA0896; hHYD
• UniProt Entry Name: UBR5_HUMAN

NCBI Description

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

UBR5: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Binds TOPBP1. Associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulates target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.

Protein type: Nuclear receptor co-regulator; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: ubiquitin binding; protein binding; zinc ion binding; RNA binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; cell proliferation; protein polyubiquitination; progesterone receptor signaling pathway; positive regulation of protein import into nucleus, translocation; protein ubiquitination during ubiquitin-dependent protein catabolic process; DNA repair; response to DNA damage stimulus