Human RIO Kinase 3 (RIOK3) ELISA Kit | RIOK3 elisa kit

Human RIO Kinase 3 (RIOK3) ELISA Kit

Cat. Num. AAA163629

• Gene Names: RIOK3; SUDD
• Reactivity: Human
• Synonyms: RIO Kinase 3 (RIOK3); Human RIO Kinase 3 (RIOK3) ELISA Kit; RIOK3 elisa kit

• Reactivity: Human
• Sequence Length: 424

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.2ng/ml - 70ng/ml
• Sensitivity: 0.07ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RIOK3 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human RIO Kinase 3 (also known as RIOK3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human RIOK3 antibody. RIOK3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RIOK3 Antibody is added and binds to RIOK3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RIOK3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human RIOK3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 112180703
• NCBI GeneID: 8780
• UniProt Accession #: O14730; Q8IXN9
• Molecular Weight: 58,671 Da
• NCBI Official Full Name: RIOK3 protein
• NCBI Official Synonym Full Names: RIO kinase 3
• NCBI Official Symbol: RIOK3
• NCBI Official Synonym Symbols: SUDD
• NCBI Protein Information: serine/threonine-protein kinase RIO3
• UniProt Protein Name: Serine/threonine-protein kinase RIO3
• UniProt Gene Name: RIOK3
• UniProt Synonym Gene Names: SUDD

NCBI Description

This gene was first identified by the similarity of its product to the Aspergillus nidulans SUDD protein. This gene is now recognized as a member of the right open reading frame (RIO) kinase gene family. This gene encodes a serine/threonine kinase that localizes to the cytoplasm and plays a role in the processing of the pre-40 S ribosomal subunit. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2017]

Uniprot Description

Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3 (PubMed:24807708). Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling (PubMed:25865883). Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway (PubMed:19557502). May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA (PubMed:22418843).