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Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using NAT1 antibody.)

Rabbit anti-Human, Mouse NAT1 Polyclonal Antibody | anti-NAT1 antibody

NAT1 Polyclonal Antibody

Gene Names
NAT1; AAC1; MNAT; NATI; NAT-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
NAT1; Polyclonal Antibody; NAT1 Polyclonal Antibody; Arylamine N-acetyltransferase 1; Arylamide acetylase 1; Monomorphic arylamine N-acetyltransferase; MNAT; N-acetyltransferase type 1; NAT-1; AAC1; anti-NAT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
NAT1 polyclonal antibody detects endogenous levels of NAT1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
290
Applicable Applications for anti-NAT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
Immunogen
Recombinant full length Human NAT1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using NAT1 antibody.)

Western Blot (WB) (Western Blot analysis of extracts of various cell lines, using NAT1 antibody.)
Related Product Information for anti-NAT1 antibody
Arylamine N-acetyltransferases (NAT-1 and NAT-2) catalyze N- or O-acetylation of heterocyclic and arylamine substrates in the detoxification of a wide array of drugs. Certain alleles causing high N-acetyltransferase activity have been associated with colon and urinary bladder cancers, as NATs also bioactivate several known carcinogens. Both NAT-1 and NAT-2 are cytoplasmic proteins and play an active role in the detoxification of many arylamine and hydrazine drugs. N-acetylation polymorphism is determined by the level of NAT activity in liver tissues, and has been linked to the action and toxicity of drugs that contain amines. Human NAT-1 is the functional homolog of rodent NAT-2, while human NAT-2 is the functional homolog of rodent NAT-1.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
9
UniProt Accession #
Molecular Weight
~34kDa
NCBI Official Full Name
Arylamine N-acetyltransferase 1
NCBI Official Synonym Full Names
N-acetyltransferase 1
NCBI Official Symbol
NAT1
NCBI Official Synonym Symbols
AAC1; MNAT; NATI; NAT-1
NCBI Protein Information
arylamine N-acetyltransferase 1
UniProt Protein Name
Arylamine N-acetyltransferase 1
UniProt Gene Name
NAT1
UniProt Synonym Gene Names
AAC1; MNAT; NAT-1
UniProt Entry Name
ARY1_HUMAN

NCBI Description

This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human genome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

NAT1: Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens. Belongs to the arylamine N-acetyltransferase family.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Acetyltransferase; Secondary Metabolites Metabolism - caffeine; EC 2.3.1.5

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: cytosol

Molecular Function: arylamine N-acetyltransferase activity

Biological Process: xenobiotic metabolic process

Research Articles on NAT1

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Product Notes

The NAT1 nat1 (Catalog #AAA3007996) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NAT1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NAT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the NAT1 nat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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