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Human PTH Intact ELISA Kit

PTH Intact (1-84) ELISA

Reactivity
Human
Synonyms
PTH Intact; PTH Intact (1-84) ELISA; PTH Intact elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
The antibodies used in the PTH ELISA were purified by affinity chromatography to be specific for well-defined regions on the PTH molecule. The peroxidase labeled antibody recognizes only the N-terminal region or the 1-34 amino acid sequence of the PTH molecule; whereas the biotinylated antibody is specific to the 39-84 segment. Accordingly, only intact PTH, which requires binding by both the enzyme conjugated and biotinylated antibodies, can be detected by this assay.
Samples
Human serum
Sensitivity
The sensitivity, or minimum detection limit, of this assay is defined as the smallest single value, which can be distinguished from zero at the 95% confidence limit. The IBL-America PTH ELISA has a calculated sensitivity of 0.9 pg/mL.
Intended Uses
The Intact-PTH ELISA is intended for the quantitative determination of Intact-PTH (Parathyroid Hormone) in human serum.
Preparation and Storage
2 - 8 degree C
Related Product Information for PTH Intact elisa kit
Principle of the assay: The Intact PTH Immunoassay is a two-site ELISA [Enzyme-Linked ImmunoSorbent Assay] for the measurement of the biologically intact 84 amino acid chain of PTH. Two different goat polyclonal antibodies to human PTH have been purified by affinity chromatography to be specific for well defined regions on the PTH molecule. One antibody is prepared to bind only the mid-region and C-terminal PTH 3 9-84 and this antibody is biotinylated. The other antibody is prepared to bind only the N-terminal PTH 1-34 and this antibody is labeled with horseradish peroxidase [HRP] for detection.In this assay, calibrators, controls, or patient samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidincoated microplate well. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stopping solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of intact PTH in the sample. A dose response curve of absorbance unit vs. concentration is generated using results obtained from the calibrators. Concentrations of intact PTH present in the controls and patient samples are determined directly from this curve.
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Product Notes

The Human PTH Intact (Catalog #AAA494652-BM7022) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494652-BM7022 ELISA Kit recognizes Human PTH Intact. It is sometimes possible for the material contained within the vial of "PTH Intact, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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