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Western Blot (WB) (All lanes : Anti-PPP2R1B Antibody at 1:1000 dilutionLane 1: HeLa whole cell lysatesLane 2: human liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit PPP2R1B Polyclonal Antibody | anti-PPP2R1B antibody

PPP2R1B Antibody

Gene Names
PPP2R1B; PR65B; PP2A-Abeta
Reactivity
Human, mouse, rat
Applications
Western Blot, ELISA, Immunocytochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PPP2R1B; Polyclonal Antibody; PPP2R1B Antibody; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta; anti-PPP2R1B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%
Concentration
1mg/ml (varies by lot)
Sequence Positions
541-600
Sequence Length
556
Applicable Applications for anti-PPP2R1B antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes
WB~~ 1:1000
Antigen Type
synthetic peptide
Antigen Source
KLH conjugated synthetic peptide derived from human PPP2R1B
Preparation and Storage
Store at -20 degree C.Stable for 12 months from date of receipt

Western Blot (WB)

(All lanes : Anti-PPP2R1B Antibody at 1:1000 dilutionLane 1: HeLa whole cell lysatesLane 2: human liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-PPP2R1B Antibody at 1:1000 dilutionLane 1: HeLa whole cell lysatesLane 2: human liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PPP2R1B antibody
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
Product Categories/Family for anti-PPP2R1B antibody
References
Baysal B.E.,et al.Gene 217:107-116(1998).
Wang S.S.,et al.Science 282:284-287(1998).
Baysal B.E.,et al.Eur. J. Hum. Genet. 9:121-129(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
66214
NCBI Official Full Name
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform isoform d
NCBI Official Synonym Full Names
protein phosphatase 2, regulatory subunit A, beta
NCBI Official Symbol
PPP2R1B
NCBI Official Synonym Symbols
PR65B; PP2A-Abeta
NCBI Protein Information
serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
UniProt Gene Name
PPP2R1B
UniProt Entry Name
2AAB_HUMAN

NCBI Description

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

Uniprot Description

PPP2R1B: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Belongs to the phosphatase 2A regulatory subunit A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 11q23.2

Cellular Component: lipid raft

Molecular Function: protein binding

Disease: Lung Cancer

Research Articles on PPP2R1B

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Product Notes

The PPP2R1B ppp2r1b (Catalog #AAA9211736) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 541-600. The PPP2R1B Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPP2R1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). WB~~ 1:1000. Researchers should empirically determine the suitability of the PPP2R1B ppp2r1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP2R1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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