Human Protocadherin-16 (DCHS1) ELISA Kit | DCHS1 elisa kit

Human Protocadherin-16 (DCHS1) ELISA Kit

Cat. Num. AAA9715799

• Gene Names: DCHS1; FIB1; MVP2; CDH19; CDH25; CDHR6; PCDH16; VMLDS1
• Reactivity: Human
• Synonyms: Protocadherin-16 (DCHS1); Human Protocadherin-16 (DCHS1) ELISA Kit; DCHS1; CDH25; FIB1; FLJ11790; KIAA1773; PCDH16; dachsous 1; fibroblast cadherin 1; fibroblast cadherin FIB1; protocadherin 16; DCHS1 elisa kit

• Reactivity: Human
• Specificity: Human Protocadherin-16 (DCHS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DCHS1. No significant cross-reactivity or interference between Human DCHS1 and analogues was observed.
• Sequence Length: 3298

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Protocadherin-16 (DCHS1) Standard Curve.)

Related Product Information for DCHS1 elisa kit

Description: This Human Protocadherin-16 (DCHS1) ELISA Kit employs a two-site sandwich ELISA to quantitate DCHS1 in samples. An antibody specific for DCHS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DCHS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCHS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCHS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: DCHS1 is a member of the cadherin superfamily whose members encode calcium-dependent cell-cell adhesion molecules. The encoded protein has a signal peptide, 27 cadherin repeat domains and a unique cytoplasmic region. This particular cadherin family member is expressed in fibroblasts but not in melanocytes or keratinocytes. The cell-cell adhesion of fibroblasts is thought to be necessary for wound healing.

NCBI and Uniprot Product Information

• NCBI GI #: 119589089
• NCBI GeneID: 8642
• UniProt Accession #: Q96JQ0
• NCBI Official Full Name: dachsous 1 (Drosophila)
• NCBI Official Synonym Full Names: dachsous cadherin-related 1
• NCBI Official Symbol: DCHS1
• NCBI Official Synonym Symbols: FIB1; MVP2; CDH19; CDH25; CDHR6; PCDH16; VMLDS1
• NCBI Protein Information: protocadherin-16
• UniProt Protein Name: Protocadherin-16
• Protein Family: Protocadherin
• UniProt Gene Name: DCHS1
• UniProt Synonym Gene Names: CDH19; CDH25; FIB1; KIAA1773; PCDH16
• UniProt Entry Name: PCD16_HUMAN

NCBI Description

This gene is a member of the cadherin superfamily whose members encode calcium-dependent cell-cell adhesion molecules. The encoded protein has a signal peptide, 27 cadherin repeat domains and a unique cytoplasmic region. This particular cadherin family member is expressed in fibroblasts but not in melanocytes or keratinocytes. The cell-cell adhesion of fibroblasts is thought to be necessary for wound healing. [provided by RefSeq, Jul 2008]