Human Protein Tyrosine Phosphatase Receptor Type F (PTPRF) ELISA Kit | PTPRF elisa kit

Human Protein Tyrosine Phosphatase Receptor Type F (PTPRF) ELISA Kit

Cat. Num. AAA2025479

• Gene Names: PTPRF; LAR; BNAH2
• Reactivity: Human
• Synonyms: Protein Tyrosine Phosphatase Receptor Type F (PTPRF); Human Protein Tyrosine Phosphatase Receptor Type F (PTPRF) ELISA Kit; PTPR-F; LAR; Leukocyte common antigen related; PTPRF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Tyrosine Phosphatase Receptor Type F (PTPRF).; No significant cross-reactivity or interference between Protein Tyrosine Phosphatase Receptor Type F (PTPRF) and analogues was observed.
• Sequence Length: 262

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.31ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Receptor Type F (PTPRF) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Tyrosine Phosphatase Receptor Type F (PTPRF) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTPRF elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase Receptor Type F (PTPRF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase Receptor Type F (PTPRF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase Receptor Type F (PTPRF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Tyrosine Phosphatase Receptor Type F (PTPRF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PTPRF elisa kit

Signal transduction; Metabolic pathway; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 78191531
• NCBI GeneID: 5792
• Molecular Weight: 211,687 Da
• NCBI Official Full Name: protein tyrosine phosphatase receptor type F
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, F
• NCBI Official Symbol: PTPRF
• NCBI Official Synonym Symbols: LAR; BNAH2
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase F
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase F
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRF
• UniProt Synonym Gene Names: LAR; LAR
• UniProt Entry Name: PTPRF_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PTPRF: Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase). Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.1.3.48; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: integral to plasma membrane

Molecular Function: heparin binding; transmembrane receptor protein tyrosine phosphatase activity; protein complex binding; protein tyrosine phosphatase activity

Biological Process: cell migration; cell adhesion; transmembrane receptor protein tyrosine phosphatase signaling pathway

Disease: Breasts And/or Nipples, Aplasia Or Hypoplasia Of, 2