Mouse CK16 Monoclonal Antibody | anti-CK16 antibody

CK16 Mouse Monoclonal Antibody

Cat. Num. AAA9400130

• Gene Names: KRT16; K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Affinity purification using immunogen.
• Synonyms: CK16; Monoclonal Antibody; CK16 Mouse Monoclonal Antibody; CK-16; cytokeratin 16; Cytokeratin-16; FNEPPK; anti-CK16 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: 6F6
• Specificity: The CK16 Mouse Monoclonal antibody detects endogenous CK16 proteins.
• Purity/Purification: Affinity purification using immunogen.
• Form/Format: Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 473

• Applicable Applications for anti-CK16 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:200
• Target Name: CK16
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(IHC staining of human gullet cancer tissue with CK16 mouse mAb(6F6) diluted at 1:200.)

Related Product Information for anti-CK16 antibody

Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.

Product Categories/Family for anti-CK16 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 24430192
• NCBI GeneID: 3868
• NCBI Accession #: NP_005548.2
• NCBI GenBank Nucleotide #: NM_005557.3
• UniProt Accession #: P08779; P30654; Q16402; Q9UBG8; A8K488
• Molecular Weight: 51,268 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 16
• NCBI Official Synonym Full Names: keratin 16, type I
• NCBI Official Symbol: KRT16
• NCBI Official Synonym Symbols: K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
• NCBI Protein Information: keratin, type I cytoskeletal 16
• UniProt Protein Name: Keratin, type I cytoskeletal 16
• UniProt Gene Name: KRT16
• UniProt Synonym Gene Names: KRT16A; CK-16; K16
• UniProt Entry Name: K1C16_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]

Uniprot Description

K16: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K6. K16 and K17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoskeleton; intermediate filament; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: epidermis development; intermediate filament cytoskeleton organization and biogenesis; cytoskeleton organization and biogenesis; keratinocyte migration; hair cycle; keratinocyte differentiation; cell proliferation; keratinization; innate immune response; morphogenesis of an epithelium; inflammatory response; negative regulation of cell migration; aging

Disease: Palmoplantar Keratoderma, Nonepidermolytic, Focal; Pachyonychia Congenita 1