Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein spinster homolog 2 (SPNS2) Standard Curve.)
Human Protein Spinster Homolog 2 (SPNS2) ELISA Kit | SPNS2 elisa kit
Human Protein Spinster Homolog 2 (SPNS2) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein spinster homolog 2 (SPNS2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein spinster homolog 2 (SPNS2) Standard Curve.)
Background: SPNS2 contains 548 amino acids and shares significant homology with zebrafish Spns2. Kawahara et al. (2009) showed that zebrafish Spns2, which contains 12 transmembrane domains, was expressed at the membrane of transfected Chinese hamster ovary cells. Overexpression of either zebrafish or human SPNS2 increased the release of sphingosine-1-phosphate (S1P) into the culture medium. Kawahara et al. (2009) concluded that SPNS2 functions as an S1P transporter in S1P secretion.an N-ethyl-N-nitrosurea-induced arg153-to-ser mutation in zebrafish Spns2 led to cardiac bifida. Expression of human SPNS2, but not human SPNS1, reversed the defect. The defect was also rescued by S1P injection or by reintroduction of zebrafish Spns2 in extraembryonic tissue yolk syncytial layer.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transporter of sphingosine 1-phosphate, a secreted lipid that is important in cardiovascular, immunological, and neural development. Defects in this gene are a cause of early onset progressive hearing loss. [provided by RefSeq, Jul 2016]
Uniprot Description
SPNS2: Sphingolipid transporter required for migration of myocardial precursors. Transports sphingosine 1-phosphate (S1P), a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. Mediates the export of S1P from cells in the extraembryonic yolk syncytial layer (YSL), thereby regulating myocardial precursor migration. Belongs to the major facilitator (TC 2.A.1) superfamily. Spinster (TC 2.A.1.49) family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 17p13.2
Cellular Component: integral to membrane; lysosomal membrane; vesicle
Molecular Function: sphingolipid transporter activity
Biological Process: B cell homeostasis; lipid transport; locomotion; lymph node development; regulation of eye pigmentation; regulation of humoral immune response; sphingolipid metabolic process; T cell homeostasis; transmembrane transport
Research Articles on SPNS2
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Product Notes
The Human SPNS2 spns2 (Catalog #AAA9713997) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713997 ELISA Kit recognizes Human SPNS2. It is sometimes possible for the material contained within the vial of "Protein Spinster Homolog 2 (SPNS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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