Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein spinster homolog 3 (SPNS3) Standard Curve.)
Human Protein Spinster Homolog 3 (SPNS3) ELISA Kit | SPNS3 elisa kit
Human Protein Spinster Homolog 3 (SPNS3) ELISA Kit
Gene Names
SPNS3; SLC62A3
Reactivity
Human
Synonyms
Protein Spinster Homolog 3 (SPNS3); Human Protein Spinster Homolog 3 (SPNS3) ELISA Kit; SPNS3; MGC29671; spinster homolog 3; SPNS3 elisa kit
Reactivity
Human
Specificity
Human Protein spinster homolog 3 (SPNS3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPNS3. No significant cross-reactivity or interference between Human SPNS3 and analogues was observed.
Sequence Length
385
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein spinster homolog 3 (SPNS3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein spinster homolog 3 (SPNS3) Standard Curve.)
Related Product Information for SPNS3 elisa kit
Description: This Human Protein spinster homolog 3 (SPNS3) ELISA Kit employs a two-site sandwich ELISA to quantitate SPNS3 in samples. An antibody specific for SPNS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPNS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPNS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPNS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: By searching a human database for sequences similar to the SLC22 family of anion transporters, Jacobsson et al. (2007) identified SPNS3. The deduced 512-amino acid protein has 12 putative transmembrane domains. Quantitative real-time PCR detected high expression of rat Spns3 in coronal brain sections of the olfactory bulb and frontal cortex. Expression was moderate in other coronal sections and in thymus, kidney, and spleen. Little to no expression was detected in other tissues examined. Jacobsson et al. (2007) determined that the SPNS3 gene contains 12 exons.Jacobsson et al. (2007) stated that the SPNS3 gene maps to chromosome 17p13.2; the mouse Spns3 gene maps to chromosome 11qB4.
Background: By searching a human database for sequences similar to the SLC22 family of anion transporters, Jacobsson et al. (2007) identified SPNS3. The deduced 512-amino acid protein has 12 putative transmembrane domains. Quantitative real-time PCR detected high expression of rat Spns3 in coronal brain sections of the olfactory bulb and frontal cortex. Expression was moderate in other coronal sections and in thymus, kidney, and spleen. Little to no expression was detected in other tissues examined. Jacobsson et al. (2007) determined that the SPNS3 gene contains 12 exons.Jacobsson et al. (2007) stated that the SPNS3 gene maps to chromosome 17p13.2; the mouse Spns3 gene maps to chromosome 11qB4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
SPNS3 protein
NCBI Official Synonym Full Names
sphingolipid transporter 3 (putative)
NCBI Official Symbol
SPNS3
NCBI Official Synonym Symbols
SLC62A3
NCBI Protein Information
protein spinster homolog 3
UniProt Protein Name
Protein spinster homolog 3
Protein Family
UniProt Gene Name
SPNS3
UniProt Entry Name
SPNS3_HUMAN
Uniprot Description
Function: Sphingolipid transporter
By similarity.
Subcellular location: Membrane; Multi-pass membrane protein
Potential.
Sequence similarities: Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family. [View classification]
Research Articles on SPNS3
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Product Notes
The Human SPNS3 spns3 (Catalog #AAA9713998) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713998 ELISA Kit recognizes Human SPNS3. It is sometimes possible for the material contained within the vial of "Protein Spinster Homolog 3 (SPNS3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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