Human Proprotein Convertase Subtilisin/Kexin Type 5 (PCSK5) ELISA Kit | PCSK5 elisa kit

Human Proprotein Convertase Subtilisin/Kexin Type 5 (PCSK5) ELISA Kit

Cat. Num. AAA451559

• Gene Names: PCSK5; PC5; PC6; PC6A; SPC6
• Reactivity: Human
• Synonyms: Proprotein Convertase Subtilisin/Kexin Type 5 (PCSK5); Human Proprotein Convertase Subtilisin/Kexin Type 5 (PCSK5) ELISA Kit; PC5; PC6; PC6A; SPC6; Subtilisin/kexin-like protease PC5; Proprotein convertase 5; PCSK5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PCSK5. No significant cross-reactivity or interference between PCSK5 and analogues was observed.
• Sequence Length: 1860

• Samples: Human tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PCSK5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PCSK5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCSK5 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PCSK5 in human tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCSK5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PCSK5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PCSK5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PCSK5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 299523015
• NCBI GeneID: 5125
• NCBI Accession #: NP_001177411.1
• NCBI GenBank Nucleotide #: NM_001190482.1
• UniProt Accession #: Q92824; Q13527; Q96EP4; F5H2G7
• Molecular Weight: 101,649 Da
• NCBI Official Full Name: proprotein convertase subtilisin/kexin type 5 isoform PC6B preproprotein
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 5
• NCBI Official Symbol: PCSK5
• NCBI Official Synonym Symbols: PC5; PC6; PC6A; SPC6
• NCBI Protein Information: proprotein convertase subtilisin/kexin type 5
• UniProt Protein Name: Proprotein convertase subtilisin/kexin type 5
• Protein Family: Proprotein convertase subtilisin/kexin
• UniProt Gene Name: PCSK5
• UniProt Synonym Gene Names: PC5; PC6; PC5; PC6; hPC6

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER. It then sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. This encoded protein is widely expressed and one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It mediates posttranslational endoproteolytic processing for several integrin alpha subunits and is thought to process prorenin, pro-membrane type-1 matrix metalloproteinase and HIV-1 glycoprotein gp160. Alternative splicing results in multiple transcript variants, some of which encode distinct isoforms, including a protease packaged into dense core granules (PC5A) and a type 1 membrane bound protease (PC5B). [provided by RefSeq, May 2014]

Uniprot Description

Likely to represent a widespread endoprotease activity within the constitutive and regulated secretory pathway. Capable of cleavage at the RX(K/R)R consensus motif. Plays an essential role in pregnancy establishment by proteolytic activation of a number of important factors such as BMP2, CALD1 and alpha-integrins.