Human Proprotein Convertase Subtilisin/Kexin Type 5 (PCSK5) RTU ELISA Kit | PCSK5 rtu elisa kit

Human Proprotein Convertase Subtilisin/Kexin Type 5 (PCSK5) ELISA Kit

Cat. Num. AAA4501696

• Gene Names: PCSK5; PC5; PC6; PC6A; SPC6
• Reactivity: Human
• Synonyms: Proprotein Convertase Subtilisin/Kexin Type 5 (PCSK5); Human Proprotein Convertase Subtilisin/Kexin Type 5 (PCSK5) ELISA Kit; PC5; PC6; PC6A; SPC6; Subtilisin/kexin-like protease PC5; Proprotein convertase 5; PCSK5 rtu elisa kit

• Reactivity: Human
• Sequence Length: 1860

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCSK5 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCSK5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PCSK5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PCSK5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PCSK5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 299523015
• NCBI GeneID: 5125
• NCBI Accession #: NP_001177411.1
• NCBI GenBank Nucleotide #: NM_001190482.1
• UniProt Accession #: Q92824; Q13527; Q96EP4; F5H2G7
• Molecular Weight: 101,649 Da
• NCBI Official Full Name: proprotein convertase subtilisin/kexin type 5 isoform PC6B preproprotein
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 5
• NCBI Official Symbol: PCSK5
• NCBI Official Synonym Symbols: PC5; PC6; PC6A; SPC6
• NCBI Protein Information: proprotein convertase subtilisin/kexin type 5
• UniProt Protein Name: Proprotein convertase subtilisin/kexin type 5
• Protein Family: Proprotein convertase subtilisin/kexin
• UniProt Gene Name: PCSK5
• UniProt Synonym Gene Names: PC5; PC6; PC5; PC6; hPC6

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER. It then sorts to the trans-Golgi network where a second autocatalytic event takes place and the catalytic activity is acquired. This encoded protein is widely expressed and one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It mediates posttranslational endoproteolytic processing for several integrin alpha subunits and is thought to process prorenin, pro-membrane type-1 matrix metalloproteinase and HIV-1 glycoprotein gp160. Alternative splicing results in multiple transcript variants, some of which encode distinct isoforms, including a protease packaged into dense core granules (PC5A) and a type 1 membrane bound protease (PC5B). [provided by RefSeq, May 2014]

Uniprot Description

Serine endoprotease that processes various proproteins by cleavage at paired basic amino acids, recognizing the RXXX[KR]R consensus motif. Likely functions in the constitutive and regulated secretory pathways. Plays an essential role in pregnancy establishment by proteolytic activation of a number of important factors such as BMP2, CALD1 and alpha-integrins.