Human Prokineticin Receptor 1 (PKR1) ELISA Kit | PKR1 elisa kit

Human Prokineticin Receptor 1 (PKR1) ELISA Kit

Cat. Num. AAA454984

• Gene Names: PROKR1; ZAQ; PKR1; GPR73; PK-R1; GPR73a
• Reactivity: Human
• Synonyms: Prokineticin Receptor 1 (PKR1); Human Prokineticin Receptor 1 (PKR1) ELISA Kit; PROKR1 PKR-1 GPR73 GPR-73a ZAQ PK-R1 G Protein-Coupled Receptor 73 G-protein coupled receptor ZAQ; PKR1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PKR1. No significant cross-reactivity or interference between PKR1 and analogues was observed.
• Sequence Length: 393

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.228ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PKR1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PKR1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKR1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PKR1 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PKR1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PKR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PKR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PKR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20373179
• NCBI GeneID: 10887
• NCBI Accession #: NP_620414.1
• NCBI GenBank Nucleotide #: NM_138964.3
• UniProt Accession #: Q8TCW9; Q53QT9; Q8NFJ7; A5JUU2
• Molecular Weight: 44,770 Da
• NCBI Official Full Name: prokineticin receptor 1
• NCBI Official Synonym Full Names: prokineticin receptor 1
• NCBI Official Symbol: PROKR1
• NCBI Official Synonym Symbols: ZAQ; PKR1; GPR73; PK-R1; GPR73a
• NCBI Protein Information: prokineticin receptor 1
• UniProt Protein Name: Prokineticin receptor 1
• Protein Family: V-type ATPase assembly factor
• UniProt Gene Name: PROKR1
• UniProt Synonym Gene Names: GPR73; PKR1; PK-R1

NCBI Description

This gene encodes a member of the G-protein-coupled receptor family. The encoded protein binds to prokineticins (1 and 2), leading to the activation of MAPK and STAT signaling pathways. Prokineticins are protein ligands involved in angiogenesis and inflammation. The encoded protein is expressed in peripheral tissues such as those comprising the circulatory system, lungs, reproductive system, endocrine system and the gastrointestinal system. The protein may be involved in signaling in human fetal ovary during initiation of primordial follicle formation. Sequence variants in this gene may be associated with recurrent miscarriage. [provided by RefSeq, Aug 2016]

Uniprot Description

Receptor for prokineticin 1. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. May play a role during early pregnancy.