Human Probable crossover junction endonuclease EME2 ELISA Kit | EME2 elisa kit
Human Probable crossover junction endonuclease EME2 (EME2) ELISA Kit
Gene Names
EME2; SLX2B; gs125
Reactivity
Human
Synonyms
Probable crossover junction endonuclease EME2; Human Probable crossover junction endonuclease EME2 (EME2) ELISA Kit; EME2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human EME2. No significant cross-reactivity or interference between Human EME2 and analogues was observed.
Sequence Length
379
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for EME2 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EME2 in samples. An antibody specific for EME2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEME2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EME2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EME2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,446 Da
NCBI Official Full Name
probable crossover junction endonuclease EME2
NCBI Official Synonym Full Names
essential meiotic structure-specific endonuclease subunit 2
NCBI Official Symbol
EME2
NCBI Official Synonym Symbols
SLX2B; gs125
NCBI Protein Information
probable crossover junction endonuclease EME2
UniProt Protein Name
Probable crossover junction endonuclease EME2
Protein Family
UniProt Gene Name
EME2
NCBI Description
EME2 forms a heterodimer with MUS81 (MIM 606591) that functions as an XPF (MIM 278760)-type flap/fork endonuclease in DNA repair (Ciccia et al., 2007 [PubMed 17289582]).[supplied by OMIM, Mar 2008]
Uniprot Description
EME2: Interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures. Belongs to the EME1/MMS4 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Deoxyribonuclease; EC 3.1.22.-
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: nuclear chromatin
Molecular Function: crossover junction endodeoxyribonuclease activity; DNA binding
Biological Process: double-strand break repair; intra-S DNA damage checkpoint; replication fork processing; resolution of meiotic recombination intermediates
Research Articles on EME2
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Product Notes
The Human EME2 eme2 (Catalog #AAA281546) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281546 ELISA Kit recognizes Human EME2. It is sometimes possible for the material contained within the vial of "Probable crossover junction endonuclease EME2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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